海藻酸钠水凝胶包封卵母细胞结晶性质研究

林春燕; 张宇琪; 周新丽

doi:10.12465/j.issn.0253-4339.2025.01.157

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海藻酸钠水凝胶包封卵母细胞结晶性质研究

更新时间：2025-01-23

- 海藻酸钠水凝胶包封卵母细胞结晶性质研究
- Crystallization Properties of Oocytes Encapsulated in Sodium Alginate Hydrogel
- 制冷学报 2025年46卷第1期页码：157-166
- 作者机构：
  
  上海理工大学生物系统热科学研究所　上海　 200093
- 作者简介：
  
  周新丽，女，教授，上海理工大学生物系统热科学研究所，13817547878，E-mail：zjulily@163.com。研究方向：低温生物医学。
- 基金信息：
- DOI：10.12465/j.issn.0253-4339.2025.01.157
  中图分类号： TB61⁺1;R318.52
- 收稿日期：2023-10-25，
  
  修回日期：2023-11-25，
  
  录用日期：2024-01-18，
  
  纸质出版日期：2025-02-16
- 稿件说明：
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林春燕, 张宇琪, 周新丽. 海藻酸钠水凝胶包封卵母细胞结晶性质研究[J]. 制冷学报, 2025,46(1):157-166.

Lin Chunyan, Zhang Yuqi, Zhou Xinli. Crystallization Properties of Oocytes Encapsulated in Sodium Alginate Hydrogel[J]. Journal of refrigeration, 2025, 46(1): 157-166.
林春燕, 张宇琪, 周新丽. 海藻酸钠水凝胶包封卵母细胞结晶性质研究[J]. 制冷学报, 2025,46(1):157-166. DOI： 10.12465/j.issn.0253-4339.2025.01.157.

Lin Chunyan, Zhang Yuqi, Zhou Xinli. Crystallization Properties of Oocytes Encapsulated in Sodium Alginate Hydrogel[J]. Journal of refrigeration, 2025, 46(1): 157-166. DOI： 10.12465/j.issn.0253-4339.2025.01.157.

摘要

基于海藻酸钠水凝胶的微囊化技术可用于优化冷冻和复温程序，降低细胞和组织的冷冻损伤。观察了卵母细胞在不同体积分数（0.5%、1.0%、1.5%、2.0%）海藻酸钠水凝胶中的形态，确定其封装的安全浓度；利用低温显微镜系统研究了不同体积分数海藻酸钠水凝胶的结晶温度及结晶行为，并对比卵母细胞在基础溶液、保护剂溶液中降/复温时形态和结晶情况；对比溶剂置换型和物理混合型制备的海藻酸钠抗冻水凝胶包封卵母细胞的冻存效果。结果表明：卵母细胞在0.5%和1.0%体积分数的海藻酸钠凝胶中整体形态与体积维持较好；1.0%体积分数海藻酸钠组和12.5%DMSO+12.5%EG+0.5 mol/L海藻糖的保护剂溶液组中的卵母细胞在降温过程中均未产生胞内冰；相比物理混合型，溶剂置换型组水凝胶中的卵母细胞在降温过程中无胞内冰产生，细胞复温后仍保持正常形态。

Abstract

Microencapsulation technology based on sodium alginate hydrogels can be used to optimize freezing and rewarming procedures and to reduce cryo-damage to cells and tissues. This study first observed the morphology of oocytes of sodium alginate hydrogels at different volume fractions (0.5%

1.0%

1.5%

and 2.0%) to determine the safe concentration for their encapsulation. Second

the crystallization temperature and crystallization behavior of sodium alginate hydrogels with different volume fractions were systematically investigated using cryo-microscopy

and the morphology and crystallization of oocytes were compared when they were cooled down/retempered in the base solution (cryoprotectant solution). Finally

the freezing effects of the sodium alginate antifreeze hydrogel-encapsulated oocytes prepared by solvent replacement and physical mixing were compared. The results revealed that oocytes maintained their overall morphology and volume better in sodium alginate gels at volume fractions of 0.5% and 1.0%. Furthermore

oocytes in both the 1.0% sodium alginate group and the cryoprotectant solution group of 12.5% DMSO + 12.5% EG + 0.5 mol/L trehalose did not produce intracellular ice during the cooling process. Additionally

compared with the physical mixture

oocytes in the hydrogel solvent replacement group did not produce intracellular ice during the cooling process

and the cells retained their normal morphology after rewarming.

关键词

Keywords

references

MIAO Shu , GUO Chenxi , JIANG Ze , et al . Development of an open microfluidic platform for oocyte one-stop vitrification with cryotop method [J ] . Biosensors , 2022 , 12 ( 9 ): 766 .

王文杰 , 葛雷 , 张树山 , 等 . 线粒体靶向抗氧化剂MitoQ在猪卵母细胞冷冻保存中的应用 [J ] . 上海农业学报 , 2020 , 36 ( 2 ): 82 - 88 .

( WANG Wenjie , GE Lei , ZHANG Shushan , et al . Application of mitochondrial targeted antioxidant MitoQ in cryopreservation of porcine oocytes [J ] . Acta Agriculturae Shanghai , 2020 , 36 ( 2 ): 82 - 88 .)

AL-AZAWI T , TAVUKCUOGLU S , KHAKI A A , et al . Cryopreservation of human oocytes, zygotes, embryos and blastocysts: a comparison study between slow freezing and ultra rapid (vitrification) methods [J ] . Middle East Fertility Society Journal , 2013 , 18 ( 4 ): 223 - 232 .

林玉婷 , 马翔 , 蔡令波 , 等 . 玻璃化冷冻的卵母细胞在卵母细胞捐赠中的应用 [J ] . 国际生殖健康/计划生育杂志 , 2016 , 35 ( 5 ): 362 - 364 .

( LIN Yuting , MA Xiang , CAI Lingbo , et al . Application of human vitrified oocytes in ovum donation [J ] . Journal of International Reproductive Health/Family Planning , 2016 , 35 ( 5 ): 362 - 364 .)

DE SANTIS L , NOTTOLA S A , COTICCHIO G , et al . Type of protein supplement in cryopreservation solutions impacts on the degree of ultrastructural damage in frozen-thawed human oocytes [J ] . Cryobiology , 2020 , 95 : 143 - 150 .

史蕾 , 马玲 , 覃菊玲 , 等 . 人成熟卵母细胞玻璃化冻融后经供精单精子显微注射受精并单囊胚冻融移植成功妊娠分娩 [J ] . 中国优生与遗传杂志 , 2022 , 8 ( 12 ): 2262 - 2266 .

( SHI Lei , MA Ling , QIN Juling , et al . Successful pregnsncy and delivery after human mature vitrification oocytes were fertilized by intracytoplasmic sperm injection and frozen-thawed single blastocysts [J ] . Chinese Journal of Birth Health & Heredity , 2022 , 8 ( 12 ): 2262 - 2266 .)

姚志芳 , 冯宇哲 , 王磊 , 等 . 卵母细胞冷冻保存技术的研究进展 [J ] . 青海畜牧兽医杂志 , 2019 , 49 ( 5 ): 58 - 61 .

( YAO Zhifang , FENG Yuzhe , WANG Lei , et al . Research progress of cryopreservation technology of oocytes [J ] . Chinese Qinghai Journal of Animal and Veterinary Sciences , 2019 , 49 ( 5 ): 58 - 61 .)

EDGAR D H , GOOK D A . A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos [J ] . Human Reproduction Update , 2012 , 18 ( 5 ): 536 - 554 .

PEREIRA B C , ORTIZ I , DORADO J M , et al . Effect of permeable cryoprotectant-free vitrification on DNA fragmentation of equine oocyte-cumulus cells [J ] . Reproduction in Domestic Animals , 2019 , 54 ( Suppl. 3 ): 53 - 56 .

CASCIANI V , MONSEUR B , CIMADOMO D , et al . Oocyte and embryo cryopreservation in assisted reproductive technology: past achievements and current challenges [J ] . Fertility and Sterility , 2023 , 120 ( 3 ): 506 - 520 .

SIMÓ G , FERNÁNDEZ-FERNÁNDEZ E , VILA-CRESPO J , et al . Research progress in coating techniques of alginate gel polymer for cell encapsulation [J ] . Carbohydrate Polymers , 2017 , 170 : 1 - 14 .

袁晓露 , 李宝霞 , 黄雅燕 , 等 . 海藻酸钠微囊的制备及应用进展 [J ] . 化工进展 , 2022 , 41 ( 6 ): 3103 - 3112 .

( YUAN Xiaolu , LI Baoxia , HUANG Yayan , et al . Progress in preparation and application of sodium alginate microcapsules [J ] . Chemical Industry and Engineering Progress , 2022 , 41 ( 6 ): 3103 - 3112 .)

CHEN Wanyu , SHU Zhiquan , GAO Dayong , et al . Sensing and sensibility: single-islet-based quality control assay of cryopreserved pancreatic islets with functionalized hydrogel microcapsules [J ] . Advanced Healthcare Materials , 2016 , 5 ( 2 ): 223 - 231 .

BELLICH B , BORGOGNA M , CARNIO D , et al . Thermal behavior of water in micro-particles based on alginate gel [J ] . Journal of Thermal Analysis and Calorimetry , 2009 , 97 ( 3 ): 871 - 878 .

ZHAO Gang , LIU Xiaoli , ZHU Kaixuan , et al . Hydrogel encapsulation facilitates rapid-cooling cryopreservation of stem cell-laden core-shell microcapsules as cell-biomaterial constructs [J ] . Advanced Healthcare Materials , 2017 , 6 ( 23 ): 201700988 .

SERRA M , CORREIA C , MALPIQUE R , et al . Microencapsulation technology: a powerful tool for integrating expansion and cryopreservation of human embryonic stem cells [J ] . PLoS One , 2011 , 6 ( 8 ): e23212 .

BIAN Jiang , LI Tao , DING Chenhui , et al . Vitreous cryopreservation of human preantral follicles encapsulated in alginate beads with mini mesh cups [J ] . The Journal of Reproduction and Development , 2013 , 59 ( 3 ): 288 - 295 .

ZHANG Hengtong , CHENG Junqiu , AO Qiang . Preparation of alginate-based biomaterials and their applications in biomedicine [J ] . Marine Drugs , 2021 , 19 ( 5 ): 264 .

CHANG C C , SHAPIRO D B , NAGY Z P . The effects of vitrification on oocyte quality [J ] . Biology of Reproduction , 2022 , 106 ( 2 ): 316 - 327 .

杨波 , 刘宝林 , 沈力 , 等 . 低温保护剂热物性分析与冰晶的显微研究 [J ] . 制冷学报 , 2014 , 35 ( 3 ): 39 - 44 .

( YANG Bo , LIU Baolin , SHEN Li , et al . Analysis of thermal and crystallization in cryoprotective agents [J ] . Journal of Refrigeration , 2014 , 35 ( 3 ): 39 - 44 .)

AUGST A D , KONG H J , MOONEY D J . Alginate hydrogels as biomaterials [J ] . Macromolecular Bioscience , 2006 , 6 ( 8 ): 623 - 633 .

LEVY G , SCHWARZ T W . The role of residual calcium in the viscosity changes of sodium alginate solutions [J ] . Journal of the American Pharmaceutical Association (Scientific Ed.) , 1958 , 47 ( 6 ): 455 - 457 .

ZHANG Wujie , YANG Geer , ZHANG Aili , et al . Preferential vitrification of water in small alginate microcapsules significantly augments cell cryopreservation by vitrification [J ] . Biomedical Microdevices , 2010 , 12 ( 1 ): 89 - 96 .

HUANG Haishui , CHOI J K , RAO Wei , et al . Alginate hydrogel microencapsulation inhibits devitrification and enables large-volume low-CPA cell vitrification [J ] . Advanced Functional Materials , 2015 , 25 ( 44 ): 6939 - 6850 .

CAGOL N , BONANI W , MANIGLIO D , et al . Effect of cryopreservation on cell-laden hydrogels: comparison of different cryoprotectants [J ] . Tissue Engineering Part C, Methods , 2018 , 24 ( 1 ): 20 - 31 .

CHEN Fan , ZHOU Dan , WANG Jiahui , et al . Rational fabrication of anti-freezing, non-drying tough organohydrogels by one-pot solvent displacement [J ] . Angewandte Chemie , 2018 , 57 ( 22 ): 6568 - 6571 .

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