| 摘要: |
| Cryotop法是玻璃化保存卵母细胞较有效的方法之一。当Cryotop载体插入液氮时，降温速度极高，几乎瞬间完成，常规的方法无法测量。实验使用数字示波器、线径25μm 的T型热电偶建立起高速测温系统，测量了Cryotop法用于卵母细胞低温保存时的降温速率。通过改变载体材料、厚度、冷源温度等条件，提高Cryotop的降温速率。结果发现当使用60μm的铜质载板，使用浆状液氮作为冷源时，降温速度最高，可达到37130±1336 K/min，较商品化的Cryotop的降温速度（11982±1936 K/min）提高了2倍。 |
| 关键词: Cryotop 降温速率 高速测温 |
| DOI： |
| Received:September 27, 2012 |
| 基金项目:国家自然科学基金（50906057,51076108)资助项目 |
|
| Cooling Rate Measurement and System Optimization for Cryotop Device |
|
Li Weijie1, Zhou Xinli1, Dai Jianjun2, Zhang Defu2, Liu Baolin1, Wang Haisong1
|
| (1.Institute of Biothermal Technology, University of Shanghai for Science and Technology;2.Animal and Veterinary Research Institute，SAAS，Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding) |
| Abstract: |
| Cryotop is one of the best effective methods for oocytes cryopreservation. When Cryotop is inserted into liquid nitrogen, the cooling speed is extremely high, which is difficult to measure by conventional method. In this study, digital oscilloscopes and type T thermocouple of 25 μm diameter were employed to establish a high speed temperature measurement system. The cooling rates of Cryotop device at different carrier material, thickness and cold source temperature were measured. The results showed that when 60μm copper was used as carrier and slush nitrogen was used as cold source, the cooling rate was increased. It could reach 37130 ±1336 K/min, which was twice higher than that of commercially available Cryotop (11982 ± 1936 K/min). |
| Key words: Cryotop cooling rate ultra-fast temperature measurement |
