| 摘要: |
| 卵巢组织冻存是女性生育力保存的重要方法，慢速冷冻卵巢组织卵泡存活率不高，自体移植效率低。本文通过诱发成核对卵巢组织降温程序进行优化，分析了成核温度和成核后降温速率对卵巢组织冷冻保存效果的影响。将程序降温仪与超声波诱发成核设备结合，实现超声波诱发成核并优化了超声波强度，对复温后的卵巢存活率和组织学进行评估。结果表明：引入诱发成核的降温程序对卵巢组织损伤减小，当在-11℃诱发成核，成核后降温速率为1℃/min时，卵泡存活率达88.02%。超声波成核设备可对样品进行无接触式诱发成核，降低了污染风险，提高了成核的成功率，冻存卵巢组织卵泡存活率提高至88.38%。通过程序的优化以及设备的改进提高了卵巢组织冷冻保存效果，降低了冷冻保存过程中引入污染的风险，为临床上卵巢组织慢速冷冻保存提供了新的方法。 |
| 关键词: 卵巢组织 冷冻保存 慢速冷冻 超声植冰 |
| DOI： |
| 投稿时间：2023-12-09 修订日期：2024-03-18
录用日期：2024-04-02 |
| 基金项目: |
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| Study of the Effect of Ice Seeding on Slow Freezing of Ovarian Tissue |
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| Abstract: |
| Ovarian tissue cryopreservation is an important method of female fertility preservation, slow freezing of ovarian tissue has poor follicular survival and low retransplantation efficency. In this paper, the ovarian tissue cooling procedure was optimized by ice seeding, and the effects of ice seeding temperature and cooling rate after seeding on the ovarian tissue cryopreservation effect were analyzed. The programmed cooling apparatus was combined with an ultrasonic device to achieve ultrasonic seeding of ice crystals, and ultrasonic intensity was screened. Ovarian survival and histology were assessed after rewarming. The results showed that the optimized cooling procedure with ice seeding reduced the damage to ovarian tissues。When ice seeding was triggered at -11°C with a cooling rate of 1°C/min after nucleation, follicle survival was 88.02%. Ultrasonic nucleation equipment allowed contactless ice seeding of the samples, which reduced the risk of contamination and improved the success rate of ice seeding, and the follicle survival rate of frozen ovarian tissue increased to 88.38%. The optimization of the procedure as well as the improvement of the equipment improved the effect of ovarian tissue cryopreservation, reduced the risk of introducing contamination during the cryopreservation process, and provided a new method for the slow cryopreservation of ovarian tissues in the clinic. |
| Key words: ovarian tissue cryopreservation slow freezing ultrasonic ice seeding |
