| 摘要: |
| 固体表面玻璃化冷冻方法（SSV）及其变式在细胞冻存领域应用越来越广泛，但多基于实验和经验，缺乏仿真分析和物理机理解释，影响相关技术的通用化设计和研发效率。本文以质量分数35%的丙二醇溶液为对象，考虑冷冻过程的非等温结晶特性，建立了耦合非等温结晶源项的SSV方法传热模型，研究了预冷温度、样品体积和形状对实施SSV方法的液体温度与结晶特性的影响。结果表明：该模型可以有效揭示冷冻过程中液滴样品内部的温度及结晶度变化规律。样品结晶危险区位于远离低温表面处，且最易结晶点不一定位于液滴顶点。预冷温度越低，冷冻样品体积越小，形状越扁平，样品降温速率越快，越容易实现玻璃化冷冻。 |
| 关键词: 细胞冻存 固体表面玻璃化冷冻 预冷 快速降温 非等温结晶 |
| DOI： |
| 投稿时间：2023-02-05 修订日期：2023-03-22
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| 基金项目:国家重点研发计划（2022YFC2703000）；上海市科委创新行动计划（20S31903400）；上海交通大学“交大之星”计划医工交叉研究基金（YG2021QN86）资助。 |
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| Numerical Study on Cooling and Crystallization Characteristics of Solid Surface Vitrification Method |
|
Zhu Wenxin1, Zhao Zhihui1, Huang Yonghua1, Cheng Jinsheng2, Chen Wei3, Li Zheng3
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| (1.School of Mechanical Engineering, Shanghai Jiao Tong University;2.Shanghai Tofflon Medical Equipment Co., Ltd.;3.Department of Andrology, Urologic Medical Center, Shanghai General Hospital, Shanghai Jiao Tong University) |
| Abstract: |
| Solid surface vitrification (SSV) has been increasingly applied in the field of cell cryopreservation. However, most studies based on empirical experimentations have not included simulation analysis and physical mechanism explanation, consequently affecting the universality of the designs and innovation efficiency in SSV-related technologies. This study focused on the non-isothermal crystallization characteristics and established a thermal diffusion model of SSV coupled with a non-isothermal crystallization source. A propylene glycol solution with the mass fraction of 35% was selected as the sample for analysis. Additionally, the effects of the pre-cooling temperature, sample volume, and sample shape on the temperature and crystallization characteristics of the SSV method were investigated. The results show that the established model can directly reveal change patterns of the temperature and the crystallinity inside the sample during the freezing process. The region most likely to undergo crystallization is located at the upper part of the sample away from the precooled surface, and the most crystallizable region is not necessarily located at the top of the droplet sample. Furthermore, vitrification of the sample is easier to achieve because of the lower precooling temperature, smaller sample volume, and flatter shape. |
| Key words: cryopreservation solid surface vitrification precool rapid cooling non-isothermal crystallization |
