| 摘要: |
| 低温冷冻保存是细胞治疗、辅助生殖等生物医学应用的使能技术之一。冷冻和复苏过程中，胞内外冰晶的形成和生长会对细胞产生损伤；其中，升降温速率又与冰晶的形成、生长有密切关系。本文在阐述程序化慢速冷冻和玻璃化快速冷冻两种细胞冻存方式及冷冻损伤两因素假说的基础上，对细胞特性、细胞外环境和升降温策略三个影响细胞冻存效果的要素进行分析，从细胞层面、细胞外环境层面和升降温策略及载体设备层面对目前细胞冻存优化技术及设备进行分类，重点梳理了细胞冻存优化在升降温策略及载体设备层面的进展，并总结了优化细胞冻存的挑战与前景。 |
| 关键词: 低温保存 冷冻损伤 玻璃化 冰晶 微流体 |
| DOI： |
| 投稿时间：2021-08-30 修订日期：2021-09-28
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| 基金项目:上海市科委创新行动计划(20S31903400)；上海市申康促进创新能力三年行动计划(SHDC2020CR3077B) |
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| Advances on Optimization Strategies and Devices for Cell Cryopreservation |
|
Zhao Zhihui, Huang Yonghua
|
| (School of Mechanical Engineering, Shanghai Jiao Tong University) |
| Abstract: |
| Cryopreservation technology has enabled biomedical applications such as cell-based therapies and assisted reproductive technology. Intracellular and extracellular ice formation and growth can lead to freezing injuries. The warming and cooling rates are closely related to the formation and growth of ice. This review first introduces two typical cryopreservation methods, namely slow programmable freezing and vitrification, and the two-factor damage mechanism. Three factors influencing cell cryopreservation are discussed. Accordingly, advances in optimization strategies and devices are classified into three categories. In detail, advances in warming and cooling strategies and related devices have been discussed. Finally, the challenges and perspectives are examined. |
| Key words: cryopreservation freezing injury vitrification ice crystal microfluid |
