摘要: |
为了研究羟基磷灰石(HA)纳米微粒对不同种类低温保护剂[渗透性丙三醇(60%,w/w)和非渗透性PEG-600(50%,w/w)]反玻璃化结晶的影响,利用低温显微镜研究了含有不同粒径(20nm、40nm、60nm)和不同质量浓度(0.1%、0.5%)HA纳米微粒的丙三醇和PEG-600溶液在反玻璃化过程中的结晶现象。实验结果表明:丙三醇和PEG-600溶液在反玻璃化过程中的冰晶形貌分别为枝状和球形,且形貌都不随添加纳米微粒而改变。与未添加纳米微粒的丙三醇溶液相比,添加60nm、0.5%纳米微粒溶液的析晶分数仅为未添加溶液的1/7。PEG-600溶液在-64℃~-52℃区间内的析晶分数显著增加,其中添加40nm、0.5%纳米微粒的PEG-600溶液的析晶分数增加了90%。冰晶的生长速率存在两个极大值现象,而添加40nm、0.5%纳米微粒的丙三醇溶液、未添加和添加60nm纳米微粒的PEG-600溶液的冰晶生长速率都只有一个极大值。 |
关键词: 低温显微镜,反玻璃化,冰晶形貌,冰晶分数,冰晶生长速率 |
DOI: |
投稿时间:2012-09-19
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基金项目:国家自然科学基金(51076108)和教育部新世纪优秀人才计划(07-0559)资助项目 |
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Effects of Hydroxyapatite Nanoparticles on Devitrification crystallization of Different kinds of Cryoprotectants |
Lv Fukou, Liu Baolin, Li Weijie
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(Institute of Biothermal and Technology, University of Shanghai for Science and technology) |
Abstract: |
To study the effects of Hydroxyapatite (HA) nanoparticles on devitrification of different cryoprotectant solutions, the crystallization of Glycerol(60%,w/w) and PEG-600(50%,w/w) solutions with HA nanoparticles of different particle size(20nm、40nm、60nm)and concentrations(0.1%、0.5%)were observed under a cryomicroscope. Experimental results showed that the morphology of Glycerol and PEG-600 solutions is dendritic and spheric respectively, and both structures are not changed by adding nanoparticles. The ice fraction of Glycerol solution containing 0.5% HA with the particle size of 60nm is 1/7 of that of Glycerol solution without nanoparticles. The ice fraction of PEG-600 solution increased drastically between -64℃ and -52℃, and the ice fraction of PEG-600 solution containing 0.5% HA with the particle size of 40nm increased by 90%. Ice growth rate has two maximum normally, however, there is only one maximum in Glycerol solution containing 0.5% HA with the particle size of 40nm, PEG-600 solution with the particle size of 60nm, and PEG-600 solution without nanoparticles. |
Key words: cryomicroscope,devitrification,morphology,ice fraction,ice growth rate |