Gut microbiota plays an important role in the health of humans and animals. The preservation quality of the fecal samples has a direct impact on the results of study. This paper summarizes the main application of fecal bacteria preservation

introduces the mechanism of cryopreservation

and summarizes the preservation methods of fecal bacteria

including cryopreservation

freeze-drying preservation

4℃ refrigerated storage and other non-cryogenic preservation methods. This paper shows that the samples frozen at -80 ℃ have a relatively stable DNA and microbial communities. We propose that the addition of an effective cryoprotectant can prevent the formation of ice crystals and increase the activity of fecal bacteria. This review discusses the main factors affecting the preservation of fecal bacteria (such as sample storage temperature

time

species types

homogenization

etc.)

and prospects some new feasible methods.