Ice crystal formation is one of the factors that causes cell damage during crvopreservation process．Ice crystal will provide fundamental information for the development of appropriate cryopreservation protocols．Effects of concentration and properties of solutions for the growth and shape of ice crystals were studied by using a cryomicoscopy system

change on the solution of phase transition was analysis by the use of differential scanning calorimetry (DSC) instrument. The experiment of cryomicoscopy demonstrates that the shape and size of the crystal of CPA are related to the concentration of the solution and the addition of the solute

small ice crystals cause little damage to the cells with the high concentration

and reduces physical damage to the cellular structure with an appropriate ratio of sorbitol and Me2SO. Furthermore

DSC demonstrates that the mixture of 5%Me2SO and 0.4Msorbitol for cryopreservation achieved a satisfactory outcome

the temperature

the enthalpy

the crystal were even better than other three solution. Therefore the injury of cells has slightest.