加拿利海枣种子的低温贮藏特性研究

吉爱玲; 何兰兰; 刘宝林

doi:10.12465/j.issn.0253-4339.2025.06.160

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加拿利海枣种子的低温贮藏特性研究

更新时间：2025-12-03

- 加拿利海枣种子的低温贮藏特性研究
- Studies on Low Temperature Storage Characteristics of Canary Date Seeds (Phoenix Canariensis)
- 制冷学报 2025年46卷第6期页码：160-166
- 作者机构：
  
  上海理工大学健康科学与工程学院　上海　 200093
- 作者简介：
  
  刘宝林，男，博士，教授，上海理工大学科技发展研究院院长，15001768141，E-mail：blliuk@163.com。研究方向：低温生物医学工程、食品冷冻冷藏等。
- 基金信息：
- DOI：10.12465/j.issn.0253-4339.2025.06.160
  中图分类号： TB661;S379.2;S339.3⁺2
- 收稿：2024-12-12，
  
  修回：2024-12-25，
  
  录用：2025-02-24，
  
  纸质出版：2025-12-16
- 稿件说明：
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吉爱玲, 何兰兰, 刘宝林. 加拿利海枣种子的低温贮藏特性研究[J]. 制冷学报, 2025,46(6):160-166.

Ji Ailing, He Lanlan, Liu Baolin. Studies on Low Temperature Storage Characteristics of Canary Date Seeds (Phoenix Canariensis)[J]. Journal of Refrigeration, 2025, 46(6): 160-166.
吉爱玲, 何兰兰, 刘宝林. 加拿利海枣种子的低温贮藏特性研究[J]. 制冷学报, 2025,46(6):160-166. DOI： 10.12465/j.issn.0253-4339.2025.06.160.

Ji Ailing, He Lanlan, Liu Baolin. Studies on Low Temperature Storage Characteristics of Canary Date Seeds (Phoenix Canariensis)[J]. Journal of Refrigeration, 2025, 46(6): 160-166. DOI： 10.12465/j.issn.0253-4339.2025.06.160.

摘要

为研究加拿利海枣种子、离体种胚的低温耐性及低温贮藏过程中的各项生理变化，将新鲜加拿利海枣种子干燥至25%、20%和15%含水量后，分别在-20 ℃和-196 ℃液氮低温保存。结果表明：完整种子和离体种胚的存活率均随含水量的下降而逐渐上升，干燥至11.6%含水量的种子和种胚生长率与未冷冻组无显著性差异。液氮保存完整种子和离体种胚生活力整体强于-20 ℃保存组。低温保存后，种胚的吸器部位受损程度较子叶柄部位严重，但这种损害不影响种胚的后续生长。干燥至8%含水量后，种子SOD活性较低于新鲜种子，CAT活性和MDA含量上升。低温保存后，种子的SOD活性、CAT活性均上升，MDA含量均下降，而干燥后种子MDA含量明显上升，表明过度脱水不利于加拿利海枣种子的超低温保存。加拿利海枣具有较高低温耐性，可在液氮条件下进行种质资源的长久保存。

Abstract

To investigate the low-temperature tolerance of

Phoenix canariensis

seeds and isolated seed embryos

along with the physiological changes that occur during cryopreservation

fresh seeds were dried to 25%

20%

and 15% moisture content and then cryopreserved at -20 ℃ and -196 ℃ (LN

). The results showed that the survival rate of intact seeds gradually increased with decreasing water content. The survival rate of isolated seed embryo cultures increased with decreasing water content

and the growth rates of seeds and seed embryos dried to 11.6% moisture content were not significantly different from those of the unfrozen groups. The viability of LN

-preserved intact seeds and isolated seed embryos was higher than that of the -20 ℃ preservation group. After cryopreservation

the sucker part of the seed embryos was damaged more severely than the cotyledon after low-temperature preservation; however

this damage did not affect the subsequent growth of the seed embryos. After drying to an 8% moisture content

the superoxide dismutase (SOD) activity of the seeds was lower than that of fresh seeds

and the CAT activity and MDA content increased. After cryopreservation

the SOD activity and CAT activity of the seeds increased

and the MDA content decreased

whereas the MDA content of the seeds increased significantly after drying

indicating that excessive dehydration was not conducive to seed cryopreservation.

Phoeni canariensis

seeds are highly resistant to low temperatures and can be preserved in liquid nitrogen for long periods.

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references

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