Effect of Ultrasonic Ice Seeding on Cryopreservation of L-02 Hepatocyte

Li Weijie; Song Liyong; Liu Baolin; Zong Guo

doi:10.3969/j.issn.0253-4339.2021.04.158

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Effect of Ultrasonic Ice Seeding on Cryopreservation of L-02 Hepatocyte

更新时间：2024-07-18

- Effect of Ultrasonic Ice Seeding on Cryopreservation of L-02 Hepatocyte
- Journal of Refrigeration Vol. 42, Issue 4, (2021)
- 作者机构：
  
  1. 上海理工大学生物系统热科学研究所
  2. 上海明悦医疗科技有限公司
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0253-4339.2021.04.158
  CLC：
- Published：2021，
- 稿件说明：
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LI WEIJIE, SONG LIYONG, LIU BAOLIN, et al. Effect of Ultrasonic Ice Seeding on Cryopreservation of L-02 Hepatocyte. [J]. Journal of refrigeration, 2021, 42(4).
DOI：

LI WEIJIE, SONG LIYONG, LIU BAOLIN, et al. Effect of Ultrasonic Ice Seeding on Cryopreservation of L-02 Hepatocyte. [J]. Journal of refrigeration, 2021, 42(4). DOI： 10.3969/j.issn.0253-4339.2021.04.158.

摘要

细胞溶液在慢速冷冻过程中会出现过冷现象，较大的过冷度会形成不可控的胞内冰。为减少过冷度，提高细胞存活率，本文提出一种用于L-02肝细胞植冰保存过程的超声波植冰系统，实验研究不同超声波强度、不同预冷温度和不同体积分数Me2SO对肝细胞存活率的影响。结果表明：当体积分数为0%~10%的Me2SO作为保护剂时，使用超声波植冰操作均能显著提高肝细胞冷冻存活率。当超声波强度为0.0329~0.431 6 W/cm2时，肝细胞存活率均在90%以上，组间无显著性差异(p<0.05)，超声波强度大于0.431 6 W/cm2时，肝细胞存活率显著下降；使用体积分数为5%的Me2SO在﹣7 ℃进行超声波植冰，超声波强度为0.104 1 W/cm2时，肝细胞存活率最高，达到(97±2.6)%，与新鲜组无显著性差异；超声波植冰能够显著提高肝细胞存活率，降低保护液中Me2SO用量。

Abstract

During the slow freezing process

the cell solution is super-cooled

which may cause uncontrollable intracellular ice formation. Ultrasonic ice seeding can reduce super-cooling and improve the cell survival rate. An ultrasonic ice seeding device was designed for the ice seeding preservation of L-02 hepatocytes

and the influence of different ultrasonic intensities

different pre-cooling temperatures

and different volume concentrations of Me2SO on the survival rate of hepatocytes were explored. The results show that when Me2SO with a volume concentration of 0%–10% is used as a cryoprotectant

the use of ultrasonic ice seeding operation can significantly improve the survival rate of hepatocytes during freezing. When the ultrasound intensities are 0.032 9 W/cm2~0.431 6 W/cm2

the hepatocyte survival rate is above 90%

and there is no significant difference between the groups (p<0.05). When the ultrasound intensity is greater than 0.431 6 W/cm2

the hepatocyte survival rate decreased significantly; ultrasonic ice seeding with Me2SO at a volume concentration of 5% at ﹣7 ℃ and an ultrasonic intensity of 0.1041 W/cm2 results in the highest survival rate of hepatocytes

which is (97±2.6)% and is not significantly different from the fresh group. Therefore

ultrasonic ice seeding can significantly improve the survival rate of hepatocytes and reduce the amount of Me2SO in the solution.

关键词

肝细胞过冷度低温保存超声植冰

Keywords

hepatocytesuper-coolingcryopreservationultrasonic ice seeding

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Related Institution

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上海理工大学

上海细胞治疗集团有限公司上海细胞治疗研究院

上海理工大学生物系统热科学研究所

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