| 摘要: |
| 冷冻和复温过程中的冰晶生长是影响细胞冻存成功率的关键因素，了解不同场景下的冰晶生长和抑制机理，对于辅助生殖等临床应用具有重要意义。本文研制了一套基于瞬态接触法的新型低温显微成像系统（降温速率可达1 600 K/min），基于此研究了不同过冷度下自由平板面、圆形槽道内纯水和二甲基亚砜溶液的冻结过程，对比了鼠生精小管在纯水和质量分数为10%二甲基亚砜溶液中的冰晶形成特性。结果表明：平板面上，二甲基亚砜溶液的冰晶生长过程相比于纯水具有随机性、多点生长的特征，且生长速率更为缓慢；圆形槽道内，随着过冷度的增加，纯水的冰晶生长速率近似线性增长，并呈现出细针状的冰晶形貌；随着二甲基亚砜的加入，鼠生精小管外的冰晶生长被弱化从而使其得以维持较为完好的生理形态。 |
| 关键词: 细胞冻存 结晶 冷冻保护剂 低温显微成像系统 |
| DOI： |
| Received:January 20, 2022Revised:February 27, 2022 |
| 基金项目:本文受上海市科委创新行动计划(20S31903400)、上海市申康促进创新能力三年行动计划 ( SHDC2020CR3077B)和上海交通大学医工交叉研究基金( YG2021QN86) 项目资助。 |
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| Visualization Study on Ice Formation in Water and Dimethyl Sulfoxide Solution |
|
Pan Pingan1, Zhang Hongbin1, Huang Yonghua1, Han Xia2, Chen Wei2, Li Zheng2
|
| (1.Institute of Refrigeration and Cryogenics, Shanghai Jiao Tong University;2.Department of Andrology, Center for Men' s Health, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Jiao Tong University) |
| Abstract: |
| Ice formation during freezing and rewarming processes is a key factor that affects the success rate of cell cryopreservation. The discovery of the formation and inhibition mechanism of ice crystals in various scenarios is highly significant for clinical applications, such as fertility treatments and assisted reproduction. A new cryomicroscopy system based on the instant contact method was developed, which achieved a cooling rate of up to 1600 K/min. The system was used to study the freezing process of pure water and dimethyl sulfoxide solution on a plate and in a circular channel under different supercooling conditions. The crystallization behavior of rat seminiferous tubules in pure water and 10% dimethyl sulfoxide solution was compared. Compared with pure water, the ice formation process of the dimethyl sulfoxide solution occurred more randomly at different positions on the plate with a slower growth rate. In the circular channel, the ice formed by pure water was mainly in the shape of a sharp needle, whose growth rate increased with the increase in the degree of supercooling. The ice formation outside the seminiferous tubules of rats was weakened by the addition of dimethyl sulfoxide; hence, the seminiferous tubules retained their physiological morphology. |
| Key words: cell cryopreservation ice formation cryoprotectant cryomicroscopy |
