| 摘要: |
| 基于海藻酸钠水凝胶的微囊化技术可用于优化冷冻和复温程序,降低细胞和组织的冷冻损伤。本文首先观察了卵母细胞在不同浓度(0.5%、1%、1.5%、2%)海藻酸钠水凝胶中的形态,确定其封装的安全浓度；其次利用低温显微镜系统研究了不同浓度(0.5%、1%、1.5%、2%)海藻酸钠水凝胶的结晶温度及结晶行为,并对比卵母细胞在基础溶液、保护剂溶液中降/复温时形态和结晶情况；最后,对比溶剂置换型和物理混合型制备的海藻酸钠抗冻水凝胶包封卵母细胞的冻存效果。结果表明,卵母细胞在 0.5%和 1%浓度的海藻酸钠凝胶中整体形态与体积维持较好； 1%海藻酸钠组和12.5%DMSO+12.5%EG+0.5 M 海藻糖的保护剂溶液组中的卵母细胞在降温过程中都没有产生胞内冰；相比物理混合型,溶剂置换型组水凝胶中的卵母细胞在降温过程中无胞内冰产生,细胞复温后仍保持正常形态。 |
| 关键词: 海藻酸钠 卵母细胞 微囊化 结晶温度 |
| DOI： |
| Received:October 25, 2023Revised:November 25, 2023 |
| 基金项目:本文受上海市肿瘤能量治疗技术与器械协同创新中心资助 |
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| Research on the Crystallization Properties of Oocytes Encapsulated in Sodium Alginate Hydrogel |
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| Abstract: |
| Microencapsulation technology based on sodium alginate hydrogels can be used to optimize freezing and rewarming procedures and to reduce cryo-damage to cells and tissues. In this paper, the morphology of oocytes in different concentrations (0.5%, 1%, 1.5%, and 2%) of sodium alginate hydrogels was first observed to determine the safe concentration for their encapsulation. Secondly, the crystallization temperature and crystallization behavior of sodium alginate hydrogels with different concentrations (0.5%, 1%, 1.5%, 2%) were systematically investigated using cryo-microscopy, and the morphology and crystallization of oocytes were compared when they were cooled down/retempered in the base solution, cryoprotectant solution. Finally, the freezing effect of sodium alginate antifreeze hydrogel encapsulated oocytes prepared by solvent replacement type and physical mixing type were compared. The results showed that oocytes maintained their overall morphology and volume better in sodium alginate gels at 0.5% and 1% concentrations. Furthermore, oocytes in both the 1% sodium alginate group and the cryoprotectant solution group of 12.5% DMSO + 12.5% EG + 0.5 M trehalose did not produce intracellular ice during the cooling process；Additionally, compared with the physical mixture, oocytes in the solvent replacement group of hydrogels did not produce intracellular ice during the cooling process, and the cells retained their normal morphology after rewarming. |
| Key words: Alginate Oocyte Microencapsulation Crystallization temperature |
