| 摘要: |
| 肠道菌群在人类和动物的健康方面起着越来越重要的作用，而粪便样本的保存质量对研究结果有直接影响。本文归纳了粪菌保存的主要作用，介绍了低温保存的机制，总结粪菌的保存方法，包括冷冻保存、冷冻干燥保存、冷藏保存和其他非低温保存方式，表明在-80 ℃下冷冻的样本具有较稳定的DNA和微生物群落，提出添加有效的低温保护剂能阻止冰晶形成，提高粪菌活性。探讨了影响样本保存效果的主要因素，并对未来具有可行性的新方法进行展望。 |
| 关键词: 低温保存 粪菌 肠道菌群 冷冻干燥 |
| DOI： |
| 投稿时间：2018-11-05 修订日期：2019-03-04
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| 基金项目:国家人类遗传资源共享服务平台（2005DKA21300）和上海市青年科技英才扬帆计划（18YF1416200）资助项目。 |
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| Fecal Bacteria Cryopreservation: A Review |
| Huo Xiaoyue,Jiang Pei,Liu Baolin,Liang Wei |
| (Institute of Biothermal Science and Technology, University of Shanghai for Science and Technology;Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, MOE-Shanghai Key Laboratory of Children’ s Environmental Health) |
| Abstract: |
| Gut microbiota plays an important role in the health of humans and animals. The preservation quality of the fecal samples has a direct impact on the results of study. This paper summarizes the main application of fecal bacteria preservation, introduces the mechanism of cryopreservation, and summarizes the preservation methods of fecal bacteria, including cryopreservation, freeze-drying preservation, 4℃ refrigerated storage and other non-cryogenic preservation methods. This paper shows that the samples frozen at -80 ℃ have a relatively stable DNA and microbial communities. We propose that the addition of an effective cryoprotectant can prevent the formation of ice crystals and increase the activity of fecal bacteria. This review discusses the main factors affecting the preservation of fecal bacteria (such as sample storage temperature, time, species types, homogenization, etc.), and prospects some new feasible methods. |
| Key words: cryopreservation fecal bacteria gut microbes lyophilization |
