| 摘要: |
| 再生水源热泵系统以城市再生水作为冷热源，为城市建筑制冷、供暖，而微生物污垢是影响再生水源热泵系统效率和安全的重要问题。本研究通过进行挂片实验，利用不同质量分数的杀菌剂苯扎氯铵每天浸泡污垢10 min，考察其抑垢作用。对污垢进行活性染色，并利用共聚焦激光扫描显微镜CLSM对其进行拍照，观察污垢内细胞的活性分布。最后利用Imaris软件分别统计活/死细胞所占的生物体积。结果表明，苯扎氯铵对微生物污垢具有抑制作用，能杀灭污垢内绝大部分细菌细胞，且该作用随其质量分数上升而增强。该研究可对相关抑垢技术提供指导，从而提高再生水源热泵系统的运行效率与系统安全，促进该项技术的实际应用。 |
| 关键词: 再生水源热泵系统 微生物污垢 苯扎氯铵 激光共聚焦扫描显微镜 |
| DOI： |
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| 基金项目:国家自然科学基金（51476090,50976060）和国家创新团队(51321002)资助项目。 |
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| Experimental Investigation on the Anti-fouling Effect of Benzalkonium Chloride against Biofouling in Treated Sewage Heat Pump System |
| Chang Siyuan,Jiang Shuo,Yang Qianpeng,Shi Lin |
| (Key Laboratory for Thermal Science and Power Engineering of Ministry of Education, Beijing Municipal Key Laboratory for CO2 Utilization &Reduction, Department of Thermal Engineering of Tsinghua University) |
| Abstract: |
| The treated sewage heat pump system reuses the wasted heat in the city sewage to cool and/or heat the buildings. Biofouling, as the key problem in treated sewage heat pump systems, decreases the system efficiency and security. In this study, rotary coupon experiments were conducted, and Benzalkonium chloride, a broad-spectrum and efficient bactericide, of various concentrations was used to immerse the biofouling 10 min per day. Vital dye was used to stain the biofouling and confocal laser scanning microscope (CLSM) was used to take microphotographs of the biofouling to show the viability distribution of the bacteria cells in the biofouling. Imaris was used to calculate the biovolume of live/dead cells. The results show that benzalkonium chloride inhibits the biofouling significantly by killing most of the bacteria cells, and the inhibition effect is enhanced as the concentration increases. This research obtains insight into the biofouling inhibiting techniques and promotes the practical application of the treated sewage heat pump system by increasing the system efficiency and security. |
| Key words: treated sewage heat pump system biofouling benzalkonium chloride confocal laser scanning microscope |
