| 摘要: |
| 为了研究羟基磷灰石（HA）纳米微粒对不同种类低温保护剂[渗透性丙三醇（60%,w/w）和非渗透性PEG-600（50%,w/w）]反玻璃化结晶的影响，利用低温显微镜研究了含有不同粒径（20nm、40nm、60nm）和不同质量浓度（0.1%、0.5%）HA纳米微粒的丙三醇和PEG-600溶液在反玻璃化过程中的结晶现象。实验结果表明：丙三醇和PEG-600溶液在反玻璃化过程中的冰晶形貌分别为枝状和球形，且形貌都不随添加纳米微粒而改变。与未添加纳米微粒的丙三醇溶液相比，添加60nm、0.5%纳米微粒溶液的析晶分数仅为未添加溶液的1/7。PEG-600溶液在-64℃～-52℃区间内的析晶分数显著增加，其中添加40nm、0.5%纳米微粒的PEG-600溶液的析晶分数增加了90%。冰晶的生长速率存在两个极大值现象，而添加40nm、0.5%纳米微粒的丙三醇溶液、未添加和添加60nm纳米微粒的PEG-600溶液的冰晶生长速率都只有一个极大值。 |
| 关键词: 低温显微镜，反玻璃化，冰晶形貌，冰晶分数，冰晶生长速率 |
| DOI： |
| 投稿时间：2012-09-19
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| 基金项目:国家自然科学基金（51076108)和教育部新世纪优秀人才计划（07-0559）资助项目 |
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| Effects of Hydroxyapatite Nanoparticles on Devitrification crystallization of Different kinds of Cryoprotectants |
| Lv Fukou,Liu Baolin,Li Weijie |
| (Institute of Biothermal and Technology, University of Shanghai for Science and technology) |
| Abstract: |
| To study the effects of Hydroxyapatite (HA) nanoparticles on devitrification of different cryoprotectant solutions, the crystallization of Glycerol(60%,w/w) and PEG-600(50%,w/w) solutions with HA nanoparticles of different particle size(20nm、40nm、60nm）and concentrations（0.1%、0.5%）were observed under a cryomicroscope. Experimental results showed that the morphology of Glycerol and PEG-600 solutions is dendritic and spheric respectively, and both structures are not changed by adding nanoparticles. The ice fraction of Glycerol solution containing 0.5% HA with the particle size of 60nm is 1/7 of that of Glycerol solution without nanoparticles. The ice fraction of PEG-600 solution increased drastically between -64℃ and -52℃, and the ice fraction of PEG-600 solution containing 0.5% HA with the particle size of 40nm increased by 90%. Ice growth rate has two maximum normally, however, there is only one maximum in Glycerol solution containing 0.5% HA with the particle size of 40nm, PEG-600 solution with the particle size of 60nm, and PEG-600 solution without nanoparticles. |
| Key words: cryomicroscope,devitrification,morphology,ice fraction,ice growth rate |
