| 摘要: |
| 将传统肝细胞冻存保护剂配方中的DMSO的浓度减为5%，并添加不同浓度的D-山梨醇、木糖醇或麦芽糖醇，置于-80℃的冰箱冻存两周，两周后将细胞快速复温，进行细胞存活率、24h贴壁率以及细胞形态学的检测，并与10%浓度DMSO组对照比较。结果表明， D-山梨醇、木糖醇、麦芽糖醇均对人肝细胞的低温保存有一定的保护作用，其中5%DMSO+0.4M D-山梨醇组复温后细胞的存活率、贴壁率均优于其他冻存液组，D-山梨醇与DMSO联合使用降低了冻存保护剂DMSO的浓度，表明两者有协同作用。 |
| 关键词: 医学细胞生物学 低温保存 肝细胞 糖醇 二甲基亚砜 |
| DOI： |
| 投稿时间：2011-09-23
|
| 基金项目:国家自然科学基金（51076108）和教育部新世纪优秀人才计划（07-0559）资助项目。 |
|
| Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs |
| Yang Bo,Shen Li,Huang Danhua,Liu Baolin |
| (School of medical instrument and food engineering, University of Shanghai for Science & Technology) |
| Abstract: |
| Different types of sugar alcohol is usually used as cryoprotective agents (CPAs) to preserve human hepatocytes. In this study, a new types of sugar alcohol based CPAs with low toxicity was explored by replacing Me2SO with D-sorbic alcohol, xylosic alcohol and maltol. The Me2SO concentration in CPAs was reduced to 5%. Human hepatocytes attached to plastic cover slips were treated with the above CPAs and then placed in the fridge at -80℃ for two weeks. The survival rate, 24h cell detachment and cell morphology were tested and compared with the results using 10% Me2SO as CPAs. The results show that 5% Me2SO with D-sorbic alcohol gives the best results. It is concluded that the new CPAs are effective to human hepatocytes cryopreservation. |
| Key words: medical cellular biology cryopreservation hepatocyte sugar-alcohol dimethyl-sulfoxide(Me2SO) |
