Currently

the precise linear cooling of microfluidics remains a challenge in the development and application of microfluidic technology in medicine and life sciences. To realize the linear cooling of the target area on a microfluidic chip

a thermoelectric refrigeration-based temperature control system for microfluidic chips was designed

and linear coupling between the current

cooling capacity

and microfluidic temperature was achieved through numerical simulation and experimental tests. When the thermoelectric cooler was driven by a constant current

the linearity of the cooling process of the microfluidic sample cell was only 0.598. The use of a single-function transient current significantly improved the linearity of microfluidic cooling; however

cold surpluses and inadequacies still existed

which did not meet the accuracy requirements of linear cooling. Finally

a transient current mechanism to realize the linear cooling in microfluidic thermoelectric cooler devices was obtained by coupling a variety of current waveforms. Linear cooling of microfluidic between -19–26 °C and 24–42 °C/min was realized

and the linearity was above 0.9981. The linear cooling mechanism of the thermoelectric cooler for microfluidic devices was successfully verified by experimental tests.