Establishing a high-quality biobank is very important

but the cryoprotective agent (CPA) type and concentration have a considerable influence on the cryopreservation effect. In this paper

HBL-100 human breast cells were treated with 10% Me2SO combined with trehalose (0-0.3 mol/L) and fetal bovine serum (FBS; 20%-60%) in Dulbecco’s modified Eagle’s medium (DMEM). Through gradual reduction of the temperature

the cells were cooled in a ﹣80 ℃ freezer for 4 h and then quickly transferred into liquid nitrogen. After storing for 7 days

the cells were shaken quickly at 37 ℃. The cell survival rate was assessed using the trypan blue test

cell counting kit (CCK) assay

and the attached rations after 24 hours. The results show that

compared with the control group

the effects of the trehalose and FBS are very obvious. In particular

when the trehalose concentration is constant

a higher FBS volume fraction yields a better effect. The FBS has no significant effect without trehalose. Further

when the FBS volume fraction is constant

the optimal effect is obtained for 0.2 mol/L trehalose. A high trehalose concentration may weaken the effect of the FBS. Considering the cost and other factors

the most suitable CPAs for the cryopreservation of HBL-100 human breast cells are 10% Me2SO + 40%-60% FBS + 0.2 mol/L trehalose.