最新刊期
卷 42 ， 期 4 ， 2021
- 摘要：The features of microgravity cryogenic fluid management (CFM) technologies were analyzed in this study, and existing space experiments on CFM technologies were reviewed. Based on the upgrade requirements of technology readiness levels, gravity-dependence analysis of 25 CFM techniques was performed. Moreover, several approaches to acquire low-gravity or microgravity environments are presented and their characteristics are compared herein. To realize further technique verifications, a series of valuable suggestions on space experimental scheme designs are provided. It should be noted that the properties of liquid hydrogen in space cannot be represented through experiments using other alternative cryogenic fluids. Instead, liquid hydrogen should be treated with special regard, which is different from liquid oxygen and liquid methane. In space-based test designs for liquid oxygen and liquid methane, liquid nitrogen can be used as a substitute in a convenient and safe view. However, for liquid hydrogen tests, hydrogen itself should be adopted. In addition, microgravity tests based on spacecraft platforms play an irreplaceable role in the upgrading of CFM techniques. In addition to the research on single CFM techniques, spacecraft-based test platforms should be designed in a timely manner.关键词：cryogenic propellant;boil-off control;multi-layer insulation;gas-liquid separation;microgravity1456|1484|2发布时间：2024-07-18
- 摘要：Halogenated alkanes and halogenated olefins, especially fluorine substitutes, are currently the most widely used synthetic refrigerants. With the implementation of a series of increasingly stringent environmental regulations, most optional fluorinated substances are flammable, thus limiting their scope of use in domestic and commercial scenarios. Novel flame-retardant methods need to be developed based on the combustion mechanism. An important method is to study the combustion process of pure or mixed refrigerants through the reaction mechanism and reaction path. In this study, the ignition delay, temperature and pressure changes, and typical component combustion processes of low-carbon fluoroolefins represented by R1234yf were studied, and the combustion mechanisms of R32 and R1234yf were compared. These results are consistent with the combustion models of alkanes and olefins. R1234yf’s ignition delay is higher, and the addition and capture reactions are complicated; through the study of the reaction path, it was found that the intermediate stable products of the two flammable working fluids have many overlaps. When R1234yf is 10%, at an equivalent ratio of 1.0, the combustion equilibrium temperature can be reduced by 87.5 ℃ compared to that for pure R32; when R32 is 5%, the combustion equilibrium temperature can be reduced by 21.0 ℃ compared to that for pure R1234yf. The flammability of the mixture was lower than that of any of the two components. With the addition of a small amount of R32, the ignition time was increased, and the peak of free radicals boosting the exothermic reaction was significantly reduced关键词：refrigerant;flammability;flame retardant;reaction path;mixing inhibition1465|1926|1发布时间：2024-07-18
- 摘要：The ejector is one of the key components of a transcritical CO2 ejector expansion refrigeration system, and the nozzle exit position (NXP) is the main geometric parameter. Therefore, research on the structure, performance, and internal flow mechanism of the ejector is beneficial for improving the performance of the ejector system. In this study, a visualization ejector with different NXPs was designed and manufactured. The entrainment ratio and pressure recovery ratio of the CO2 two-phase ejector with different NXPs were obtained in a transcritical CO2 ejector expansion refrigeration system. Furthermore, the pressure distribution along the primary nozzle, mixing section, and diffuser section was measured using a pressure sensor. Simultaneously, the expansion angle, expansion length, and phase change position of the primary flow were captured using a high-speed camera. According to the experimental results, the performance of the ejector is influenced by the expansion profile. Specifically, the short NXP (4 mm) led to a short expansion length, large expansion angle, and high-pressure recovery ratio, but the corresponding entrainment ratio was relatively low. As the NXP increased to 6 mm and 8 mm, the expansion of the primary flow was more sufficient, the expansion length was longer, the expansion angle was smaller, and the entrainment ratio was improved. A further increase in the nozzle distance to 10 mm contributed to a larger expansion angle as well as a low entrainment ratio and pressure recovery ratio. From the results, the optimal NXP is in the range of 6–8 mm for the ejector under the operating conditions of this study. The results of this study contribute to a better understanding of the internal flow mechanism of the ejector and help to improve its design theory.关键词：two-phase ejector;visualization;expansion profile;nozzle exit position1371|1340|1发布时间：2024-07-18
- 摘要：For finned-tube heat exchangers, owing to the extremely different refrigerant pressure drop characteristics between evaporation and condensation modes, the optimum refrigerant channel number of the evaporation mode is larger than that of the condensation mode. However, the conventional heat exchanger cannot achieve better matching characteristics between the refrigerant circuit and variations in operating conditions. To solve this problem, a vapor-bypassed evaporation technique is applied in a residential air conditioner, and experimental studies have been conducted to investigate the corresponding system performance under various operating conditions. Experimental results show the following: under heating mode, as the heating capacity ranges 2~5 kW, the vapor-bypassed evaporation system yields 2.5%~7.2% higher COP over baseline; under cooling mode, when the cooling capacity increases from 1.5 kW to 4.5 kW, the COP of vapor-bypassed evaporation system is improved by 8.6%~3.5% in comparison with the baseline; finally, according to the GB 21455—2019 Standard[1], the APF (annual performance factor) of vapor-bypassed evaporation system is improved by 6.4%.关键词：residential air conditioner;APF index;fined-tube heat exchanger;vapor-bypassed evaporation technique1202|1250|0发布时间：2024-07-18
- 摘要：To evaluate whether air conditioning energy consumption after building operations meet the requirements of an energy-saving design, lacks a unified standard owing to the different usage behaviors, which often results in deviations and miscalculations. Based on this research background, this study proposes an index of air conditioning operation degree hours by combining three factors: air conditioning set temperature, operation days, and daily operation hours. Combined with the characteristics of residential air conditioning in the Yangtze River region, the air conditioning behavior is divided into economic, comfortable, and high consumption types. The operation degree hours of typical cities and the critical values of the three models were calculated. A relative energy consumption index is proposed by comparing the actual air-conditioning operation degree hours of residences with the corresponding critical value in the air conditioning behavior mode, and the energy consumption levels can be compared with each other. Taking two residential buildings in different areas as examples, the reliability and effectiveness of the operation degree hours were verified, and the R-squared was above 0.93. The application of this evaluation method of air conditioning energy consumption in residential buildings is illustrated, and the energy consumption characteristics of air conditioning and relative energy consumption level of two cases in different regions were analyzed. The results show that the relative energy consumption indices is 9.8% and 73.1% for Residences 1 and 2, respectively. Residence 1 is more energy-efficient, excluding the influence of weather conditions and air-conditioning behavior.关键词：residential building;air conditioning usage behavior;operational degree hours;energy consumption evaluation1334|1314|0发布时间：2024-07-18
- 摘要：There are many air supply terminals in the central air-conditioning system of large-scale public buildings, and the load demand varies greatly. Although the commonly used control methods can meet the terminal load demand, their energy consumption is large. In this study, an optimal control model for the air supply and chilled water system of a central air-conditioning system was established. The energy consumption of the system is considered as the optimization goal, and the hybrid algorithm of beetle antennae search-particle swarm optimization algorithm (BAS-PSO) is used to solve this problem, which not only saves energy but also improves the defects of traditional PSO. Considering the air handling subsystem of a centralized air-conditioning system in a public building in Shanghai as an example, modeling and optimization control solutions were carried out. The results show that the maximum energy saving of BAS-PSO is 252.02 kW, and the energy saving rate is approximately 20% compared with the original control scheme, that is, constant air supply temperature control. The experimental test results show that the optimization algorithm can achieve a 14.6% energy saving rate, saving 153.15 kW of energy, which proves that the optimal control model and optimization algorithm have reliable application prospects.关键词：central air-conditioning system;optimal control;particle swarm optimization algorithm(PSO);beetle antennae search algorithm(BAS)1452|1310|0发布时间：2024-07-18
- 摘要：A novel type of gas–liquid coupled double-acting traveling-wave thermoacoustic refrigeration system is proposed to solve the low efficiency of traditional thermoacoustic refrigeration with a gas resonance tube. The study optimized the size of the system structure and analyzed its performance using numerical simulation. First, the article studied the distribution of important parameters such as sound power, pressure, and volume flow amplitude of a three-stage gas-liquid coupled double-acting traveling wave thermoacoustic refrigeration system. Then, characteristic parameters such as the acoustic power and thermoacoustic conversion efficiency of the system under different levels were investigated. Finally, the cooling power of different stages and COP with pressure were compared and analyzed. The calculation results show that when the ambient temperature is 293 K, heating temperature is 400 K, cooling temperature is 270 K, and mean pressure is 1 MPa, the COP of three-stage system reaches 0.7, the cooling power is 0.78 kW, the operating frequency is 13.8 Hz, and the relative Carnot efficiency of the system is 22.3%. As the number of system stages increases, the power density of the system increases. When the mean pressure is 10 MPa, the cooling power of the six-stage system reaches a maximum value of 16.1 kW, and the COP is 0.38.关键词：thermoacoustic;thermoacoustic engine;thermoacoustic cooler;gas-liquid coupling1463|1230|1发布时间：2024-07-18
- 摘要：In view of the uneven heat transfer and high wind resistance of traditional symmetrical louver fin heat exchangers, which are most commonly used in automobile heat pump air conditioners, six asymmetric louver fin structures were proposed. STAR-CCM+ software was used to numerically simulate the structure of asymmetric louver fins, and the change characteristics of the internal temperature and pressure fields of the heat exchanger under the corresponding four working conditions were studied, and the heat transfer factor j and resistance factor f of each fin were calculated. The results show that although the heat exchange of the new asymmetric louver fin B3 group is slightly reduced, it has a significant effect on the reduction of wind resistance. When the air inlet velocity is 4.5 m/s, the resistance factor f is reduced by 31.9%, the heat transfer of the entire fin is more uniform, and there is no obvious concentrated heat transfer part on the entire fin. This study also conducted experimental research on a new type of fin, and the results show that most of the errors between the experimental and simulation results are below 10%.关键词：parallel flow heat exchanger;asymmetrical type;structural design;numerical calculation1365|1300|0发布时间：2024-07-18
- 摘要：In order to analyze the actual heating effect and operation performance of a new type of radiant floor heating system coupled with air-source heat pump applied in the northern cold area, a 76-day field test was conducted in an office building for the management of residential area in Tianjin. During the test period, the average temperature of indoor working area was 18.4~21.8 ℃, and the average indoor surface temperature was 22.5~29.1 ℃. More than 90% of indoor LPD1 and LPD2 were in grade I index. The average daily heating capacity and power are 1.5~6.9 kW and 0.6~3.2 kW, respectively; the daily average power consumption is 0.79 kW?h/m2, and the daily average COP is 1.43~3.54. When the temperature difference between the floor surface and the the office area is within 5 ℃, the heat dissipation can still reach 50–100 W/m2. In the coldest test days, the outdoor temperature is -10.1~2.1 ℃, the average temperature in the working area of three indoor test positions is 21.4 ℃, the lowest indoor instantaneous temperature is 16.4 ℃, LPD1 and LPD2 are all in the standard range of grade I and II, which ensures the heating quality. The range of the heating capacity and power of the system is 6.1~10.9 kW and 2.9~3.3 kW, respectively, and the range of COP is 1.91~3.49.关键词：new type radiant floor;air-source heat pump;operation performance;radiant heating;field test1209|1294|1发布时间：2024-07-18
- 摘要：To meet the growing thermal management needs of data centers, immersion phase change cooling technology is adopted. This project established a performance model of an immersion phase-change cooling system and simulated the performance of four coolants in this model to confirm the effects of different coolants on its performance. The results showed that: 1) Among the four coolants, D-1 had a stronger overall performance with the widest applicable pressure range and the smallest heat flux required for startup, but the maximum heat dissipation capacity of D-1 was lower than those of FC-72 and HFE-7100. 2) As the flow rate increased, the heat carried by the cooling water tended to a stable value, and different coolants had essentially the same influence on the pressure drop of the cooling water in the tube. 3) Increasing the inlet temperature of the cooling water was beneficial for energy recovery and utilization of the outlet cooling water, but the cooling effect of the system was not good. While reducing the inlet temperature was conducive to improving the cooling capacity, it might cause problems of inefficient utilization of cold energy. 4) In the graphs, under the condition of the same inlet temperature and flow rate of cooling water, the curves of heat carried by the cooling water and temperature rise in the vapor environment of D-1 were close to or basically in line with that of Novec 649, which means that Novec 649 could be substituted by D-1 in the immersion phase change cooling system to exert a better cooling effect.关键词：data center;immersion phase change cooling system;electronic fluoride liquids;performance simulation1413|2432|3发布时间：2024-07-18
- 摘要：In a traditional temperature and humidity independent control system, low temperatures and a high relative humidity of fresh air happens when condensation dehumidification is used for fresh air treatment. In this study, a temperature-adjustable energy-saving dehumidification system with double cold sources was proposed to solve these problems. Theoretical analysis and experimental verification were conducted to study the factors affecting the supply air status, including compressor frequency, cooling coil water flow, and fresh air volume. Control devices were then developed for the supply air status in accordance with its variation. The results show that the system has a satisfactory adjustability of the comfortable supply air. Under standard conditions, the supply air temperature can be as high as 25.0 ℃. Compared with conventional dehumidification, the efficiency improvement of this new system is up to 93.6%.关键词：temperature and humidity independent control;dual cold sources;heat recovery;temperature-adjustable dehumidifier;dehumidification with condensation1342|1285|0发布时间：2024-07-18
- 摘要：To address the issues of lower thermal efficiency, susceptibility to environmental influences, and floating liquid desiccant in traditional liquid desiccant regeneration methods using packed towers, this article proposes a liquid desiccant regeneration method based on vacuum membrane distillation. The effects of liquid desiccant temperature, flow rate, mass fraction, and the vacuum degree of the system on the membrane flux, thermal efficiency, transmembrane mass transfer coefficient, and rejection rate were studied through experiments and simulations. The results show that the membrane flux increases with an increase in the liquid desiccant temperature, flow rate, and vacuum degree of the system and decreases sharply with an increase in the liquid desiccant mass fraction. The deviation of the experimental membrane flux from the simulated ones are within ±10%. The thermal efficiency increases and tends to be constant with the increase in liquid desiccant temperature and the vacuum degree of the system and decreases with an increase in the liquid desiccant flow rate and mass fraction. The transmembrane mass transfer coefficient increases with the increase in liquid desiccant temperature, flow rate, and system vacuum, and decreases with an increase in the liquid desiccant mass fraction. In the experiment, when the liquid desiccant temperature, the flow rate and the mass fraction are 80 ℃, 1.0 m/s and 20%, respectively, the vacuum degree of the system is 93 kPa, and the membrane flux reaches the maximum, which is 7.18 kg/(m2?h). Even at a high mass fraction of 40%, the membrane flux can still reach 1 kg/(m2?h). The thermal efficiency is generally higher than 80%. When the solution flow rate is 0.6 m/s, all performance parameters can reach a higher level.关键词：liquid desiccant regeneration;liquid desiccant air conditioning;vacuum membrane distillation;membrane flux;heat and mass transfer1322|1307|1发布时间：2024-07-18
- 摘要：In order to explore the new utilization of vacuum membrane distillation with fiber membrane modules in the drying field, a vacuum membrane distillation-superheated steam drying system was designed, and the changing rule of the system of membrane flux and thermal efficiency were analyzed, and the drying quality of pod pepper seeds was investigated to explore the application scenarios of the new drying process. The results show that the heat and mass transfer performance of the new drying system is similar to that of the vacuum membrane module. With the increase in feed temperature, the steam output of the system also increases with the growth rate, while the thermal efficiency increases with the growth rate. the steam flow is 41 m3/h, and the thermal efficiency is 90.6% at 70 ℃, which is 3.53% higher than that of hot air drying at 50 ℃ .The control of superheat for low-pressure superheated steam is explored, and future application prospects and research findings are discussed.关键词：vacuum membrane distillation;superheated steam;drying;degree of superheat;germination rate1368|1451|0发布时间：2024-07-18
- 摘要：Aiming at the temperature requirement of 6–7 ℃ in the field of building air conditioning cold storage, a composite inorganic salt phase change material was prepared with Na2SO4?10H2O and Na2HPO4?12H2O as cold storage base materials. The effects of different types and quality additives on the composite inorganic salt system were studied by step cooling curve analysis, differential scanning calorimetry, and the transient plane heat source method. The experimental results show that the final ratio of the modified composite inorganic salt system is 32% Na2SO4?10H2O + 48% Na2HPO4?12H2O + 16% NH4Cl + 1.6% borax + 1.6%CMC + 0.8%nano-TiO2. The phase transformation temperature is 6.1~6.3 ℃. The latent heat of the phase transformation was 130–139 J/g. The undercooling is less than 0.5 ℃, and no phase separation phenomenon exists. The liquid-phase thermal conductivity is 0.798 9 W/(m?K). After 50 cycles, the composite inorganic salt system exhibits good thermal stability.关键词：inorganic hydrated salt;air conditioning cold storage;phase change materials;preparation1143|1466|3发布时间：2024-07-18
- 摘要：In order to improve the poor thermal conductivity and long-term heat storage of paraffin as a phase change material (PCM), this study placed copper foam into a cylindrical heat storage device filled with paraffin. Three cases were designed, namely, the vertical distribution of copper foam in the radius direction of the cross section, distribution along the radius direction of the cross section, and distribution along the horizontal direction of the cross section. The influence of different layout cases on the heat transfer of paraffin-based PCM and the heat release characteristics of paraffin/metal copper foam composites were studied. The experimental results show that the addition of copper foamed metal shortens the solidification time of paraffin. The solidification time of case 1 and case 3 is shortened by five minutes, and the solidification time of case 2 was shortened by four minutes. Simultaneously, the temperature uniformity of the paraffin at different positions was improved. The temperature fluctuation of case 1 in the outer ring measurement point is 33% and 50% of those of cases 2 and 3, respectively. The effective heat release of Case 1 is 2.2% higher than that of Case 2, and the heat release efficiency increases by 5.05%. The effective heat release of Case 1 is 1.1% higher than that of Case 3, and the heat release efficiency increases by 2.01%. The heat transfer efficiency of case 1 is maintained at more than 70%, and the heat transfer effect with cold fluid is the best, which is the optimal layout case.关键词：phase change heat exchange;foamed metal copper;layout mode;heat release rate1282|1159|0发布时间：2024-07-18
- 摘要：The active gas consumption technology of a gas bearing is adopted to realize oil-free lubrication and non-contact operation of a linear compressor to ensure the reliability of the compressor. To study the influence of the structural parameters of the porous bearings and the design parameters of the compressor on the gas consumption, this study used R600a as the refrigerating medium to establish a porous gas bearing model, and the gas consumption was simulated and calculated using FLUENT. The influence of the porous material thickness, thickness of the gas film, discharge pressure, operating frequency, and cooling capacity ratio on the gas consumption and gas consumption rate of the gas bearings was analyzed, and the accuracy of the model was verified through experimental tests. The results show that the error between the simulation result and the experimental measurement of gas bearing gas consumption is within ±15%. The optimal design parameter combination is given according to the gas consumption rate. This provides a reference for the optimization design of porous gas bearings for linear compressors.关键词：linear compressor;porous gas bearing;gas consumption;gas lubrication1321|1180|1发布时间：2024-07-18
- 摘要：This paper conducts an experimental study on the performance of the air cooler in stable operation. According to the ASHRAE standard, the air cooler test room was built, and the performance of the air cooler varies with factors such as the storage temperature, the driving temperature difference, and the ratio cycle was studied. The results show that when the driving temperature difference is 10 ℃ and ratio cycle is 3, the heat transfer performance of the air cooler increases with the increase of the storage temperature. When the storage temperature increases from -34 ℃ to 10 ℃ the heat transfer coefficient increases from 16 W/(m2?℃) to 18.8 W/(m2?℃), with a percentage of 17.5%; When the cycle rate is 3, as the heat transfer temperature difference increases from 6 ℃ to 10 ℃, the heat transfer coefficient changes in a small range, and the maximum change in the test range is 4.9%. When the storage temperature is -25 ℃, the heat transfer coefficient varies from 16.8 to 17.63 W/(m2?℃), when the storage temperature is -10 ℃, the heat transfer coefficient varies from 18.1 to 18.43 W/(m2?℃); with the increase of ratio cycle, the heat transfer coefficient increases accordingly.关键词：air cooler;cold storage;cycle rate;heat transfer performance1342|1361|0发布时间：2024-07-18
- 摘要：The solidification/melting model of FLUENT software was used to numerically study the influence of contact angle and mass fraction on the freezing process of pure water and sodium chloride solution on the cold surface. Meanwhile, copper was chosen as the hydrophilic surface and nanometer film as the hydrophobic surface, and the freezing process of droplets under different surface characteristics was experimentally studied. The results show that the freezing characteristics of the droplet on the surface of the plate are related to the surface contact angle and mass fraction. When the solution mass fraction is fixed, a smaller contact angle results in a faster droplet freezing rate and a shorter complete freezing time. At the beginning of the freezing process, the smaller the contact angle, the lower the bottom temperature of the droplet. When the freezing time and the droplet height were the same, the temperature and liquid fraction on the surface of the droplet were lower than those inside the droplet. At the same contact angle, the solution mass fraction is inversely proportional to the initial freezing temperature of the droplet and directly proportional to the complete freezing time. The comparison between the experimental results and the simulation results shows that the freezing time of different mass fractions of sodium chloride droplets exhibits the same trend when the contact angle is 60° and 100°, but the experimental value is greater than the simulated value.关键词：droplet;freezing;contact angle;mass fraction1391|1200|1发布时间：2024-07-18
- 摘要：In this study, a dynamic ice-making system with supercooling water was designed and built, and a visual flat-plate supercooler was developed. The influence of flow and heat transfer on the freezing behavior in the flat-plate supercooler was studied by a large number of experiments, and the mechanism was explained by the classical nucleation theory and boundary layer theory. The results showed that there were two types of freezing behavior in the flat-plate supercooler: freezing behavior 1 shows that no freezing occurs in the supercooler and the system can continuously produce ice slurry, and freezing behavior 2 shows that freezing occurs in the supercooler and the system cannot produce ice slurry. The low inlet temperature of the secondary refrigerant and too large or too small flow of ice-making solutions were largely responsible for promoting the occurrence of freezing behavior 2, which was not conducive to the ice-making stability of the system. When freezing behavior 2 occurs, freezing occurs first on the wall with a relative distance d=0.15, where the temperature is always lower than that of the wall with a relative distance d=0.25, 0.35, 0.45, so the local condition for freezing to occur first is a lower wall temperature.关键词：ice slurry;flow and heat transfer;plate heat exchanger;freezing behavior1210|1218|0发布时间：2024-07-18
- 摘要：Based on computational fluid dynamics (CFD) simulation, the research focused on the influence of different insulation structures and different temperatures of tomatoes on the uniformity, quality, and effect of tomatoes in cold storage. The tomato was used as the test object, and a three-dimensional model of the semiconductor refrigerating box was established to get the temperature field. The test results were verified and analyzed further. When the external temperature was 20 ℃, it took 30 and 35 minutes for the average temperature in the vacuum insulation panel (VIP) combined with polyurethane (PU) box and PU box, respectively, to decrease from 20.7 ℃ to 5 ℃, and the average temperature was 4.58 ℃ and 4.97 ℃, respectively, 35 minutes later, with maximum temperature differences of 6.97 ℃ and 8.09 ℃, respectively. Tomatoes that were pre-cooled to 4 ℃, 10 ℃ and 15 ℃ in cold storage were put into the two boxes to simulate delivery for 10 hours at 2.5 ℃. With the VIP+PU box and PU box, the final temperatures of these tomatoes were 2.38 ℃, 4.29 ℃, 5.89 ℃ and 3.33 ℃, 5.15 ℃, 6.67 ℃, and the uniformity of temperature in tomatoes σ was 0.52, 0.44, 0.41, 0.57, 0.47, and 0.43, respectively. The model calculation results are consistent with the experimental results. The results showed that the thermal insulation performance, cooling rate, and cooling uniformity of the VIP+PU box were considerably better than those of the PU box; 4 ℃ was the optimal initial temperature of tomato with 0.08% weight loss rate, minimal change in lightness and chroma, and 5.13% content of total soluble solids under 2.5 ℃ refrigerated environment.关键词：semiconductor refrigerating box;vacuum insulation panel;polyurethane;CFD;nutritional quality1409|1266|0发布时间：2024-07-18
- 摘要：During the slow freezing process, the cell solution is super-cooled, which may cause uncontrollable intracellular ice formation. Ultrasonic ice seeding can reduce super-cooling and improve the cell survival rate. An ultrasonic ice seeding device was designed for the ice seeding preservation of L-02 hepatocytes, and the influence of different ultrasonic intensities, different pre-cooling temperatures, and different volume concentrations of Me2SO on the survival rate of hepatocytes were explored. The results show that when Me2SO with a volume concentration of 0%–10% is used as a cryoprotectant, the use of ultrasonic ice seeding operation can significantly improve the survival rate of hepatocytes during freezing. When the ultrasound intensities are 0.032 9 W/cm2~0.431 6 W/cm2, the hepatocyte survival rate is above 90%, and there is no significant difference between the groups (p<0.05). When the ultrasound intensity is greater than 0.431 6 W/cm2, the hepatocyte survival rate decreased significantly; ultrasonic ice seeding with Me2SO at a volume concentration of 5% at ﹣7 ℃ and an ultrasonic intensity of 0.1041 W/cm2 results in the highest survival rate of hepatocytes, which is (97±2.6)% and is not significantly different from the fresh group. Therefore, ultrasonic ice seeding can significantly improve the survival rate of hepatocytes and reduce the amount of Me2SO in the solution.关键词：hepatocyte;super-cooling;cryopreservation;ultrasonic ice seeding1188|1301|1发布时间：2024-07-18
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