最新刊期
卷 42 ， 期 3 ， 2021
- 摘要：A mechanical vapor recompression (MVR) system is an efficient evaporation system. In this study, the performance of an MVR system in generating high concentration CaCl2 solutions was studied through simulation analysis and experiments. The influence of input parameters, including evaporation pressure and inlet dilute solution concentration, on the regeneration performance of the MVR system was studied. The regeneration rate is affected by evaporation pressure on which the suction density is depended. The higher the evaporation pressure, the higher the regeneration rate and energy consumption of the system. Under the condition that the concentration of the CaCl2 solution at the inlet was 0.3, the SEC value of MVR regeneration system increased from 46.67 to 79.51 kWh/m3, and the COP of the system decreased from 14.4 to 8.2 when the evaporation pressure increased from 20 to 80 kPa. The concentration of the inlet solution affects the regeneration performance of the MVR system by increasing the boiling point of the solution. The higher the concentration of the solution, the higher the boiling point, and the higher the power consumption of the compressor. Under the condition that the evaporation pressure was 20 kPa, the SEC value of the system increased from 36.68 to 75.76 kWh/m3, and the COP of the system decreased from 18.1 to 9.1 when the concentration of inlet CaCl2 solution increased from 0.25 to 0.39. In terms of solution concentration, equivalent moisture content, and evaporation pressure, the CaCl2 solution had the best regeneration performance in the MVR system compared with the LiCl and LiBr solutions.关键词：mechanical vapor recompression system;solution regeneration;calcium chloride solution;mathematical model1550|1789|2发布时间：2024-07-18
- 摘要：A stepped pressure cycle is proposed to improve the energy efficiency of refrigeration and heat pump cycles. By constructing multiple suction and discharge pressures, the refrigerant temperatures in the evaporators and condensers underwent step changes and approached the heat source temperatures. This helps to improve heat transfer efficiency and reduce the compression ratio. However, we found that the conclusion of energy savings for the stepped pressure cycle is conditional. Over 6.5% of the energy-saving potential is reduced because of inefficient heat exchangers or a small temperature glide of the heat transfer fluid. Moreover, an excessive subcooling degree will lead to a worse COP than a simple cycle. Finally, in this study, some application cases are summarized to demonstrate the energy-saving potential of the stepped pressure cycle.关键词：refrigeration;heat pump;enengy-saving;stepped pressure cycle1547|1784|0发布时间：2024-07-18
- 摘要：In this study, the working mechanism, performance evaluation indexes, and theoretical models of an ejector are summarized. The effects of the configuration and dimensions of an ejector and its nozzle, suction chamber, constant-area section, and diffuser on the performance are reviewed. Some typical examples of applications of ejector refrigeration systems are described, including single and multi-stage ejector refrigeration, combined compression–ejector refrigeration, combined ejector-absorption refrigeration, and ejector refrigeration systems without pump. Finally, prospective proposals of ejector research are suggested.关键词：ejector refrigeration;ejector;refrigeration cycle1768|1999|2发布时间：2024-07-18
- 摘要：In this study, the surface temperature of the frost layer was measured using an infrared thermal imager and calibrated using direct measurements from a micrometer and thermocouple device. The growth characteristics of the frost layer on the surface of the finned-tube heat exchanger were experimentally studied. The effects of fin type and fin pitch on the frost thickness, frost mass, and heat transfer capacity were analyzed. The frost growth characteristics were analyzed by considering the condition at the frost wet air interface as the driving force of heat and mass transfer. The experimental results indicated that the driving force of heat and mass transfer at the interface of the wavy fin and the split fin heat exchangers is higher than that of the flat fin, resulting in higher growth rates of the frost layer on the wavy and split fins than that on the flat fin. In the late frosting period, the growth rate of the frost layer on the split fin was significantly accelerated. Among the three types of fins, the frosting duration on the split fin was the shortest and the longest on the flat fin. The average heat transfer capacity of the wavy fin heat exchanger during the frosting and defrosting periods was the largest, which was 0.61% and 2.67% higher than that of the split and flat fin heat exchangers, respectively. Meanwhile, the influence of the fin pitch on the driving force of heat and mass transfer at the interface is weak, and the larger the fin pitch is, the faster the frost layer thickness increases, and the longer the frosting duration is. Considering the average heat transfer capacity during the frosting defrosting period and the air flow resistance of the heat exchanger, the optimal fin pitch is approximately 2.2 mm under the frosting condition.关键词：air source heat pump;fin type;fin pitch;frost surface temperature1645|1644|2发布时间：2024-07-18
- 摘要：A method for preparing transparent superhydrophobic surfaces was proposed, and its anti-frosting performance at cold surface temperatures -3–5℃ and relative humidity 20%–60% was investigated. The experimental results show that the maximum contact angle of the transparent superhydrophobic glass surface prepared using silicon dioxide and polydimethylsiloxane is as large as 154.1° and has very good transparency. Frosting tests under vertical natural convection conditions indicated that the modified glass surface had a good anti-frosting effect. The frost layer formed consisted of a wheat-like spike structure in the early stage and transformed into an irregular bifurcation structure in the later stage, which is different from that of the unmodified glass surface whose frost layer structure consisted of flower clusters in the early stage and hexagonal frost crystals in the later stage. The contact angle of the modified glass surface first increased and then decreased with the nano-SiO2 coating amount, that is, there exists an optimum nano-SiO2 coating amount, found to be 0.067 g/cm2 under the experimental conditions covered. This optimum surface can remain unfrosted for 700 s, which is 66.7% longer than that of an unmodified glass surface. The results also revealed that the higher the relative humidity, the more obvious the dendritic growth of the frost crystal. The directional characteristics of frost crystal growth on the transparent superhydrophobic surface became more obvious as the humidity increased.关键词：defrosting;superhydrophdbic;silicon dicxide;relative humidity1498|1759|1发布时间：2024-07-18
- 摘要：A refrigerant cireuitry qptimization method using an ineger gadient descent algorithm for hea pump finned-tube evaporators was devebped .A zone-based evaponlor simulation model was built, and the entopy genention variations with different evaporator refrigenant circuits were invetigated. It was concluded that when the designed heat transfer capacity increases. refrigerant passes should be increased, and the split point should be moved forward to minimize evaporator entropy generation. Subsequently, experiments were conducted to validate the relationship between the lower evaporator entropy generation (theoretical results) and the higher COP (experimental results) of a specific heat pump system. The evaporator entropy generation based on simulation results proved an effective parameter for predicting system COP variation trends. Subsequently, evaporator entropy generation was selected as the cost function, and an integer gradient descent algorithm was applied to minimize the evaporator entropy generation. With the proposed optimization method, the optimum evaporator refrigerant circuits corresponding to the heat transfer capacity in the range of 2000–6000 W were selected.关键词：evaporator refrigerant circuits;gradient descent algorithm;entropy generation minimization theory;optimization method1420|1758|3发布时间：2024-07-18
- 摘要：The metal foam used for filling a heat exchanger channel can effectively improve two-phase heat transfer performance and has good application prospects. Accurate prediction of the two-phase pressure drops in the metal foam channel is required for equipment and system designs in engineering applications. It is necessary to develop a suitable method for predicting the two-phase pressure drop in metal foam channels under a wide range of conditions. The two-phase pressure drop experimental data in the metal foam channels of six references were used. The experimental conditions included metal foam pores per inch (PPI) of 5–40, porosity of 0.87–95, channel hydraulic diameter of 4.36–13.8 mm, mass flux of 0–350 kg/（m2·s）,and quality of 0–0.8. The distribution of the two-phase multiplier and the Lockhart-Martinelli parameter was analyzed, and it was found that the two-phase multiplier increased with a decrease in the ratio of the metal foam pore diameter to the channel hydraulic diameter and increased with an increase in the quality; when the diameter ratio changes from 0.31 to 0179, the two-phase multiplier increases by 1.37 to 1.52; and when the quality changes from 0 to 0.8, the two-phase multiplier increases by a factor of 3.41. A new correlation was developed based on the Lockhart-Martinelli correlation. The results show that the absolute average error between the predicted values and experimental data is 22%, which indicates that the new correlation can provide an accurate prediction.关键词：metal foam;two-phase pressure drop;predictive correlation1507|1495|0发布时间：2024-07-18
- 摘要：In this study, one smooth tube and two double-sided enhanced tubes with the same size were used in an experimental study and the boiling heat transfer performance outside the tube with a near-azeotropic refrigerant (R410A) was compared. For the boiling heat transfer outside the smooth tube, the internal surface coefficients of heat transfer calculated by the Cooper and Gorenflo formulas were compared with the empirically calculated value. The results showed that the errors between the two was less than 15%, which verifies the accuracy of the experimental results. With the help of Wilson diagram method, the heat transfer enhancement ratios of the two double-sided enhanced tubes are 1.85–2.05 and 2.02–2.25, respectively. By changing the heat flux, the total heat transfer coefficients of heat exchange tube #1 and heat exchange tube 2# and surface coefficients of heat transfer on both sides of the tube were comprehensively compared. The results showed that the outer surface structure of heat exchange tube 1# was more conducive to the formation of bubbles in the vaporization core because of the different types of reinforced ribs. The total heat transfer coefficients of heat exchange tube 1# was approximately 10% higher than that of heat exchange tube 2#, and the surface coefficient of heat exchange tube 1# was approximately 20%–25% higher than that of heat exchange tube 2#. It indicates that heat exchange tube 1# is more in line with the requirements of pool boiling heat transfer enhancement for horizontal single pipe.关键词：pool boiling;R410A;heat transfer enhancement;Wilson plot method;heat transfer correlation1495|1505|1发布时间：2024-07-18
- 摘要：With the development of aeronautics and astronautics, more and more attention has paid to efficient heat transfer inside a tube. Metal foam has a large specific surface area and high thermal conductivity, which are suitable for enhancing the flow boiling heat transfer. In this study, the effect of the metal foam structure on the flow boiling heat transfer in tubes was studied experimentally. The experimental conditions covered a pore density of 10–40 pores per inch (PPI), vapor quality of 0.1–0.9, mass flux of 90–180 kg/(m2·s), and a heat flux of 12.4–18.6 kW/m2, and the wettability of the hydrophobic or uncoated surfaces. The experimental results show that the heat transfer coefficient of the 40 PPI metal foam tube is up to 96% higher than that of the of the 10 PPI metal foam tube. With an increase in vapor quality, mass flux, and heat flux, the flow boiling heat transfer in the metal foam filled tube is increased by a maximum of 74%, 95%, and 48%, respectively, and hydrophobic modification increases the number of nucleate sites on the metal foam surface, resulting in a heat transfer enhancement of 10%–30%.关键词：flow boiling;metal foam;pore density;heat transfer1437|1390|0发布时间：2024-07-18
- 摘要：Accurate chiller models are important for the energy conservation of chillers. The DOE-2 model is the most common chiller model. Parameter identification and error compensation are crucial for the development of an accurate DOE-2 model. However, the parameter identification of a DOE-2 model of an actual chiller is usually challenging because chillers are usually equipped with limited sensors and the quality of actual data is usually low. To address the above issues, a genetic algorithm based on an external knowledge base for parameter identification and artificial-neural-network-based (ANN-based) error compensation method are proposed. The results show that the proposed genetic algorithm can significantly reduce the computation load of parameter identification of the DOE-2 model. It can also significantly improve the accuracy of the DOE-2 model. Moreover, the accuracy of the DOE-2 model with the ANN-based error compensation is significantly higher than that of the DOE-2 model without error compensation. The MAE, RMSE, MAPE, and CV-RMSE of the model with error compensation in predicting the chilled water outlet temperature were reduced by 36.49%, 46.00%, 33.16%, and 45.73%, respectively, while R2 of the model with error compensation was increased by 25.75%.关键词：chiller modeling;genetic algorithm;parameter identification;artificial neural network;error compensation1464|1991|1发布时间：2024-07-18
- 摘要：As a key part of two-phase cooling systems, direct cooling plates have received increasing attention with the development of two-phase cooling technology. A parallel mini-channel direct cooling plate was designed based on the experimental system of pumped two-phase cooling. The channel area of the cold plate was 140 mm long and 50 mm wide, having 21 parallel mini-channel, each 1.5 mm × 1.5 mm, separated by a fin with a thickness of 0.5 mm. The heat transfer characteristics of a parallel mini-channel direct cooling plate under different refrigerant inlet temperatures, flow rates, and heat fluxes were investigated. The variations in the local wall temperatures and heat transfer coefficients in different regions of the cooling plate were analyzed. The results indicate that when the heat flux is 0.5 W/cm2, the inlet temperature of the refrigerant is 15℃ the flow rate is 150 kg/h, and the wall temperatures in different channels rise monotonously along the flow direction. After the heat flux was increased to 6 W/cm2, the wall temperature first increased along the flow direction and then stabilized at a certain value. The wall temperatures of the middle channel were higher than those of the peripheral channel. The local heat transfer coefficient shows little variation under low heat flux, while an increase along the flow direction can be observed near the outlet of the mini channel under higher heat flux owing to the nucleate boiling, and the maximum increase in the heat transfer coefficient was 21%. Similar to the wall temperatures, the local heat transfer coefficient of the peripheral channel is higher because of the more intense flow boiling, with a maximum increase of 24%.关键词：two-phase cooling;flow boiling heat transfer;parallel mini-channel;direct cooling plate1472|1606|0发布时间：2024-07-18
- 摘要：The uneven distribution of refrigerants is a key issue in outdoor heat exchanger research. In this study, two types of baffles were designed, and a total of seven samples were prepared and placed in the heat exchanger header for experimental research. Through experiments, the refrigerant distribution and heat transfer performance of the six working conditions of the heat exchanger as an evaporator and the four working conditions of the condenser were analyzed. The results show that using baffles achieves a good refrigerant distribution effect, and the addition of two baffles (A and B) can help improve the uneven distribution of the refrigerant in the heat exchanger and improve the heat transfer performance. In addition, the type and number of baffles and the position of the inlet and outlet of the heat exchanger have an impact on the heat transfer performance. When the heat exchanger is used as an evaporator, the increase in the number of baffles increases the refrigerant capacity of baffle A by 15.28% and baffle B by 10.87%, reducing the pressure drop of baffle A by 26.07% and baffle B by 58.82%. The rise in the inlet and outlet positions reduces the pressure drop of baffle A by 55.56% and increases the refrigerant capacity by 12.81%. The downward movement reduces the pressure drop of baffle A by 55.56% and baffle B by 130% and increases the refrigerant capacity of baffle A by 13.42% and baffle B by 4.84%. When used as a condenser, the increase in the inlet and outlet positions reduces the pressure drop of baffle A by 16.67% and baffle B by 27.03% and increases the refrigerant capacity of baffle A by 4.72% and baffle B by 3.9%.关键词：automobile air-conditioning;heat pump;outdoor heat exchanger;refrigerant distribution1540|1705|0发布时间：2024-07-18
- 摘要：Determining the power consumption of data center servers is the basis of data center energy saving and environmental control. At present, it is a common research method that uses performance testing software to fit the server power consumption model under different working conditions. An experimental platform was built with the aim of CPU computing as the main task. Three different test software (Lookbusy, Stress, and SPECpower_ssj 2008) were used to conduct the test. This study compared the differences between server power consumption and hardware utilization based on server operating parameters. The results show apparent differences in server hardware calls under an identical parameter setting among different testing software, leading to a maximum deviation of more than 15% on the testing outcome of the server power consumption. This indicates that it is important to select the proper performance testing software according to application scenarios and hardware utilization when it comes to the research of the server power consumption model. Among the software tested, SPECpower_ssj 2008 is most suitable for the comprehensive testing of servers, and Stress is most suitable for testing the stability of servers under extreme conditions.关键词：data center;server power consumption;performance testing software;CPU utilization1657|2109|0发布时间：2024-07-18
- 摘要：Based on the principle of air humidification-dehumidification, a novel household water purifier with a vapor compression refrigeration system was proposed and established in this study. The influence of the inlet water flow rate on the key parameters of the system performance was analyzed. These performance parameters included the operating conditions of the system, cooling capacity, power consumption, coefficient of performance (COP), water production rate, and water production per unit energy consumption. The results showed that the refrigerant inlet and outlet temperatures of the main components of the system (compressor, evaporator, and auxiliary condenser) and the suction and exhaust pressures of the compressor all showed a downward trend with the increase in the inlet water flow rate under experimental conditions (ambient temperature of 25 °C, circulating air volume of 100 m3/h, inlet water temperature of 25 ℃, inlet water flow rate of 2.5–4.5 L). The system power consumption also showed a downward trend, while the water production per unit energy consumption showed an upward trend. There was an optimal inlet water flow rate of 3.5 L/h when the water production rate, cooling capacity and COP of the system reached their peak values, which were 0.682 L/h, 699 W and 2.44, respectively, and the water production per unit energy consumption was 2.058 L/kWh. The hygienic indicators (i.e., TDS less than 3) and daily production (above 12 L/d) of the purified water in the system can meet the drinking requirements of general households, and the system can also produce domestic hot water not lower than 60 °C, which has wide application prospects.关键词：water purifier;vapor compression refrigeration;humidification-dehumidification;inlet water flow rate;system performance1330|1470|1发布时间：2024-07-18
- 摘要：Direct refrigerant cooling has the advantages of low cost, high cooling efficiency, low weight, and high safety, and also problems of low evaporating temperature and uneven battery temperature in normal direct cooling refrigerant systems. In this study, the effectiveness of secondary throttling on the temperature regulation of a direct cooling plate was tested and verified. The results show that the direct cooling plate outlet pressure is increased while the superheat is reduced by adding a throttling device (fixed orifice device or a pressure-regulating valve with adjustable orifice) behind the cooling plate, thereby increasing the evaporation temperature and leading to better temperature uniformity. However, the throttle device with a fixed aperture cannot actively adjust the outlet pressure of the direct cooling plate, which increases as the thermal load increases. Therefore, it is difficult to control the battery temperature within an appropriate range when the thermal load changes. The throttle device with an adjustable aperture can adjust the direct cooling plate outlet pressure to a target value according to the operation load of the battery, which can not only avoid the evaporation temperature from being too low, but also improve the temperature uniformity.关键词：electric vehicle;power battery;refrigerant direct cooling;throttling device1376|1633|1发布时间：2024-07-18
- 摘要：A new ventilation method called interactive cascade ventilation was proposed in this study to facilitate a healthy and comfortable indoor environment with low energy consumption. Interactive cascade ventilation can take full advantage of thermal buoyancy by optimizing the energy grade of mechanical ventilation and determining the temperature gradient between jets to transform the action direction of thermal buoyancy. This study investigated the performance of interactive cascade ventilation based on temperature distribution, energy utilization efficiency, and indoor thermal comfort. The research results showed that the average indoor temperature under interactive cascade ventilation increased by only 2.5℃ with a 25% reduction in cooling energy input compared with stratum ventilation. The ventilation effectiveness under interactive cascade ventilation was 1.004, which was higher than that under stratum ventilation. Moreover, thermal comfort significantly improved. The ADPI under interactive cascade ventilation was 0.852 4, while that under stratum ventilation was 0.841 7. In addition, the PMV under interactive cascade ventilation was better distributed and closer to a thermally neutral environment. Hence, interactive cascade ventilation can realize the improvement of energy application of mechanical ventilation in both quality and quantity by using different grade energies to create jets of different temperatures. It can provide new solutions to promote the creation of a non-uniform indoor environment and present important energy-saving, environmental protection, and social benefits.关键词：interactive cascade ventilation;energy use efficiency;indoor comfort;air distribution performance index1412|1623|0发布时间：2024-07-18
- 摘要：In this study, an airfoil structure was added in front of the display cabinet shelf to optimize the performance of a vertical open-type refrigerated display cabinet. The influence of the airfoil structure on the performance of a vertical open refrigerated display cabinet was studied by testing the performance of the refrigerated display cabinet after addition of the airfoil structure. The test results show that under an ambient temperature of 25 ℃and relative humidity of 60 %, the evaporation temperature of the refrigeration system increased from ?6.3 ℃ to ?4.5 ℃ after installation of the airfoil structure. The average temperature fluctuation of supply and return air varied from 0.6?4.4 ℃, 10.3?12.0 ℃ to 1.8?3.0 ℃ and 6.0?7.4 ℃. The supply air velocity was essentially the same, and the return air velocity decreased by 10.2%–16.9%. The thermal permeability coefficient in the display cases decreased by 46%–48%. The maximum average temperature difference of food in different layers of the cabinet decreased by 31%, and the maximum average temperature difference of food inside and outside decreased by 46.2%. The total heat emission of the showcase decreased from 82.33 kW?h/24 h to 66.49 kW?h/24 h. The total energy consumption of the system decreased from 42.91 kW?h/24 h to 33.61 kW?h/24 h. The results show that the refrigerating system performance, air curtain performance, temperature uniformity, and energy utilization ratio of the open display cabinet can be improved by adding an airfoil structure at the front end of the shelf.关键词：refrigerated display case;airfoil structure;air curtain;food temperature;refrigeration system;energy consumption1367|1391|0发布时间：2024-07-18
- 摘要：As the core equipment of communication and transmission technology, switches develop rapidly, and the heat flux emitted by internal chips increases constantly. Improving heat dissipation efficiency is a prerequisite for the reliable operation of data centers. This study focuses on the simulation and experiments of immersion liquid cooling technology. The effects of this technology were analyzed and evaluated through a liquid cooling simulation of high-power switches and heat dissipation efficiency experiments of immersion liquid cooling. The results show that the model correction method based on the heat dissipation simulation analysis of components improves the accuracy of temperature prediction. The component temperature of the switch in the immersion liquid cooling condition can be reduced by approximately 20 ℃ compared with the air-cooling environment under the same power. The power limit of the unit volume of the switch under immersion liquid cooling is approximately 3.2 times of that under air cooling environment.关键词：switch;immersion liquid cooling technology;thermal simulation;heat dissipation efficiency;limit power1421|1656|0发布时间：2024-07-18
- 摘要：In a mobile air conditioner with a double air duct, two nozzles were installed at the lower left and lower right corners of the condenser respectively, for spray cooling. With an enthalpy difference experiment, the temperature at different state points, energy consumption and refrigerating capacity were tested with outdoor temperatures of 35 ℃/24 ℃ and indoor temperatures of 27 ℃/19 ℃ (dry bulb temperature/ wet bulb temperature), and the influence of different spray quantities from 0.55 g/s to 2.10 g/s on the performance of mobile air conditioning was analyzed. The results show that with the increase in spray quantity, the evaporating pressure was almost unchanged at 1.0 MPa, but the condensing pressure decreased from 3.45 MPa to 3.14 MPa, and the condensing temperature decreased from 55.5 ℃to 51.3 ℃. Moreover, energy consumption decreased from 1582.95 W to 1465.1 W, refrigerating capacity increased from 3090 W to 3339 W, and the EER increased from 1.95 to 2.28. The cold exergy was small and the exergy efficiency of the system was only 5.19% to 6.08% because of a small difference of only 8 ℃ between the indoor and ambient temperatures. Among the different components, the maximum exergy loss coefficient occurred in the compressor, which was 0.496, 0.472, 0.479 and 0.476, respectively, while the lowest exergy efficiency was obtained in the evaporator, which was only 24.6%–26.0% .关键词：effect on performance;exergy;spray cooling;mobile air conditioner1398|1402|0发布时间：2024-07-18
- 摘要：In this study, an R134a closed-loop spray cooling system was built to investigate the effects of flow rate, subcooling degree, and refrigerant charge on the steady spray cooling heat transfer performance. The experimental flow rate ranged from 0.20 to 0.25 L/min, the subcooling degree ranged from 5 to 8 ℃, and the refrigerant charge ranged from 0.95 to 1.25 kg. Results show that at a flow rate of 0.184 L/min and refrigerant charge of 0.95 kg, the maximum heat flux and surface heat transfer coefficient were 105.25 W/cm2 and 2.54 W/（cm2?℃）, respectively. At low heat flux (45.93–72.55 W/cm2), with the increase in flow rate, subcooling degree, and refrigerant charge, the surface heat transfer coefficient, overall, increased. Under higher heat flux (84.02–105.25 W/cm2), the surface heat transfer coefficient increases gradually with the increase in flow rate. The surface heat transfer coefficient initially increased and then stabilized as the refrigerant charge increased. In addition, the Jacob number Ja decreases with an increase in charge, which is unfavorable for the improvement of the surface heat transfer coefficient at higher heat flux. There is an optimal refrigerant charge to maximize the heat transfer performance of the closed spray cooling system.关键词：spray cooling;heat transfer performance;subcooling degree;refrigerant charge1459|1528|4发布时间：2024-07-18
- 摘要：Three-dimensional in vitro models constructed using cell spheroids or cell aggregates have gradually become more suitable in the field of tumor research. However, cryopreservation methods for cell spheroids are not ideal. The influence of the seeding density of A549 cells and cell culture days on the size and growth of A549 spheroids was studied. The results showed that when the seeding density was 2×104 cells /mL and the cells were cultured for three days, cell spheroids were in good condition and the microaggregates formed a compact structure with a diameter of 344.24 ± 0.74 μm, which is most suitable for cryopreservation. Then, the cooling box and program control cooling instrument were used to cryopreserve the A549 spheroids. After rewarming, the vitality and proliferation rate of cell spheroids were much lower than those of fresh cell spheroids. Plastic straws and glass capillaries were used to quickly freeze or vitrify the A549 spheroids. When glass capillaries were used as the freezing carrier and 20% EG + 20% Me2SO + 0.5 mol/L trehalose was used as the cryoprotectant, the relative vitality of A549 spheroids to fresh spheroids was 94.59% ± 9.23%, and the three-day culture proliferation rate was closest to that of the fresh group. Finally, the cooling rate of the freezing carrier and the critical cooling rate of the cryoprotectant were measured, and the thermodynamic mechanism of cell spheroid cryopreservation was analyzed.关键词：lung cancer cell spheroids;slow freezing method;vitrification;cryoprotectant1149|1356|0发布时间：2024-07-18
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