Research Progresses in Vitrification by Minimum Volume Method

Zhang Xiaomin; Zhou Xinli

doi:10.3969/j.issn.0253-4339.2018.04.063

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Research Progresses in Vitrification by Minimum Volume Method

更新时间：2024-07-18

- Research Progresses in Vitrification by Minimum Volume Method
- Journal of Refrigeration Vol. 39, Issue 4, (2018)
- 作者机构：
  
  上海理工大学医疗器械与食品学院
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0253-4339.2018.04.063
  CLC：
- Published：2018
- 稿件说明：
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Zhang Xiaomin, Zhou Xinli. Research Progresses in Vitrification by Minimum Volume Method[J]. Journal of refrigeration, 2018, 39(4).
DOI：

Zhang Xiaomin, Zhou Xinli. Research Progresses in Vitrification by Minimum Volume Method[J]. Journal of refrigeration, 2018, 39(4). DOI： 10.3969/j.issn.0253-4339.2018.04.063.

摘要

在低温保存领域，玻璃化能有效防止冰晶的形成，具有良好的保存效果。玻璃化保存需要的条件是极快的降温速率和高浓度的低温保护剂，但是高浓度低温保护剂会对细胞造成渗透损伤和毒性损伤。最小体积法能通过减小样本体积，大大提高降温速率，降低实现玻璃化所需的保护剂浓度。本文综述了最小体积法玻璃化保存的最新研究进展，包括有载体的微滴玻璃化保存、喷射微滴玻璃化保存、生物打印微滴玻璃化保存和微流体封装微滴玻璃化保存。其中，喷射微滴玻璃化保存，生物打印微滴玻璃化保存及微流体封装微滴玻璃化保存，是将新型的微滴产生技术与玻璃化技术相结合，因其能迅速产生大小均匀的微滴，具有高通量特点，在低温保存领域具有很大的发展潜力。

Abstract

Vitrification is an effective method in the field of cryopreservation since it can prevent the formation of ice crystals. Vitrification requires ultra-fast cooling rates and high concentrations of cryoprotective agents. However

osmotic injury and toxic damage can be caused by high concentration of cryoprotective agents. The minimum volume methods can significantly increase the cooling rate and reduce the concentration of cryoprotective agents in vitrification. In this paper

the latest research progresses in vitrification by minimum volume methods are reviewed. The minimum volume methods include carrier-based droplet vitrification

spray droplet vitrification

bio-printing droplet vitrification

and microfluidic encapsulation droplet vitrification. Among these

spray droplet vitrification

bio-printing droplet vitrification

and microfluidic encapsulation droplet vitrification are the combination of emerging droplet generation technology and vitrification technology. Because of the rapid generation of uniform droplets and high throughput

they have great potential in the field of cryopreservation.

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Related Institution

Institute of Biothermal Science, University of Shanghai for Science and Technology

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Yang Ge Zhang Shaozhi Chen Guangming Yu Xiaoyi Wu Dongbo(Institute of Refrigeration , Cryogenics,Zhejiang University

Institute of Biothermal Technology, University of Shanghai for Science and Technology

School of Mechanical Engineering, Shanghai Jiao Tong University

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