Freeze-drying is a long-term and effective method for cell preservation. The lyoprotectant can have a significant effect on cell freeze-drying. In this study

different volume concentrations of Me2SO

glycerol

and different mass concentrations polyvinylpyrrolidone (PVP)

compound lyoprotectant for lyophilizing human Hepatoma Hep-G2 cells were considered. The effect of intracellular trehalose on the lyophilized cells was investigated by determining the cell-recovery rate

survival rate

and 24 h attachment rate. The results show that

compared with the control group

the cells added in a 40% PVP (w/v) + 10% (v/v) glycerol + 15% fetal bovine serum (FBS) (v/v) + 20% trehalose (w/v) solution received better protection. The cell recovery rate

survival rate

and 24 h adherent rate are 29.58%

42.18%

and 18.71%

respectively. When the extracellular trehalose molar concentration is 800 mmol/L

the Hepatoma Hep-G2 cell-recovery rate

survival rate

and 24 h adherent rate are 27.81%

66.65%

and 33.68%

respectively. It is significantly better than the control group and has the best protection for cell freeze-drying.