Effects of Cryopreservation on Apoptosis in Hep-G2 Cells

Liang Wei; Yao Lan; Liu Baolin

doi:10.3969/j.issn.0253-4339.2017.01.113

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Effects of Cryopreservation on Apoptosis in Hep-G2 Cells

更新时间：2024-07-18

- Effects of Cryopreservation on Apoptosis in Hep-G2 Cells
- Journal of Refrigeration Vol. 38, Issue 1, (2017)
- 作者机构：
  
  上海理工大学生物系统热科学研究所
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0253-4339.2017.01.113
  CLC：
- Published：2017
- 稿件说明：
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Liang Wei, Yao Lan, Liu Baolin. Effects of Cryopreservation on Apoptosis in Hep-G2 Cells[J]. Journal of refrigeration, 2017, 38(1).
DOI：

Liang Wei, Yao Lan, Liu Baolin. Effects of Cryopreservation on Apoptosis in Hep-G2 Cells[J]. Journal of refrigeration, 2017, 38(1). DOI： 10.3969/j.issn.0253-4339.2017.01.113.

摘要

样本质量是体现生物样本库价值的关键，但是低温保存可能导致细胞凋亡及基因表达量的变化，影响样本质量。本文采用流式细胞术与实时荧光定量PCR（qPCR）的方法，研究了Hep-G2细胞在不同条件下冻存后凋亡及相关基因表达量。结果表明：低温保存影响Hep-G2细胞的成活率、凋亡情况及某些凋亡基因表达量。不论冻存过程中是否添加低温保护剂，低温保存都会对细胞的凋亡相关基因表达带来不同程度的影响。不添加保护剂冻存会导致细胞死亡，添加10% DMSO冻存会导致细胞凋亡，复苏培养24 h后细胞的成活率、凋亡情况与相关基因表达量基本与对照组水平一致。

Abstract

The quality of biospecimens is a very important factor of evaluating a biobank. Cryopreservation may induce apoptosis and changes of gene expression in cells. In this research the apoptosis profiles were assessed by Flow Cytometry and the expression of RNAs related to apoptosis were estimated by Real-time fluorescent quantitative PCR (qPCR) in Hep-G2 cells after cryopreservation. The results showed that with the addition of 10% DMSO or no cryoprotectant during cryopreservation

the cryopreservation progress made some impact on apoptosis and the expression of RNAs related to apoptosis in Hep-G2 cells. During cryopreservation

the cells were all dead with no cryoprotectant

but more cells apoptosis occurred with 10% DMSO. The cells could be recovered to normal level when the cells were recultured for 24 h after cryopreservation.

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Related Institution

Institute of Biothermal Science and Technology, University of Shanghai for Science and Technology

上海理工大学

上海细胞治疗集团有限公司上海细胞治疗研究院

上海理工大学生物系统热科学研究所

Yang Ge Zhang Shaozhi Chen Guangming Yu Xiaoyi Wu Dongbo(Institute of Refrigeration , Cryogenics,Zhejiang University

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