Yang Yun, Zhou Xinli, Dai Jianjun, et al. Study on Injuries of Porcine MII-stage Oocytes during Cryoprotectants Addition and Removal Processes[J]. Journal of refrigeration, 2016, 37(5).
DOI:
Yang Yun, Zhou Xinli, Dai Jianjun, et al. Study on Injuries of Porcine MII-stage Oocytes during Cryoprotectants Addition and Removal Processes[J]. Journal of refrigeration, 2016, 37(5). DOI: 10.3969/j.issn.0253-4339.2016.05.106.
Study on Injuries of Porcine MII-stage Oocytes during Cryoprotectants Addition and Removal Processes
Cryoprotectants addition and removal are essential steps during oocytes cryopreservation
however
high concentration of cryoprotectants may cause osmotic and toxic injuries to oocytes. In order to study the injuries of porcine MII-stage oocytes during cryoprotectants addition and removal processes
cryoprotectant was loaded and unloaded to/from oocytes with traditional methods (one-step method
two-step method)
the effects of vitrification solution
thawing solution and dilution solution on the survival rate of oocytes were investigated. The experimental results showed that the osmotic injuries to oocytes happened mainly in two stages: the transfer process from vitrification solution to thawing solution
and the cryoprotectants removal process. The concentration of thawing solution and equilibrium time had a great influence on the survival rate of oocytes. The cryoprotectants removal process was highly correlated with addition process
and the selection of thawing solution concentration and equilibrium time relied on the vitrification solution concentration and equilibrium time. These results could provide a reference for cryopreservation protocols of porcine MII-stage oocytes.
Osmotic Injury to Porcine Oocytes during Cryoprotective Agents Addition and Removal Processes
Evaluation of Cell Permeability Characteristics and Damage during Natural Evaporation of Cryoprotectant-Loaded Microdroplets
Experimental Study on a Novel Solution for Cryopreservation of Human Umbilical Cord Mesenchymal Stem Cells
Crystallization Properties of Oocytes Encapsulated in Sodium Alginate Hydrogel
Related Author
Teng Yun
占太杰
刘林峰
崔梦冬
何伟
胥义
Liu Linfeng
Xu Yi
Related Institution
上海理工大学
Institute of Biothermal Science and Technology, University of Shanghai for Science and Technology, Shanghai Technical Service Platform for Cryopreservation of Biological Resources, Shanghai Collaborative Innovation Center for Tumor Treatment with Energy
Institute of Biothermal and Technology, University of Shanghai for Science and Technology
Institute of Biothermal Technology, University of Shanghai for Science and Technology
Institute of Biothermal Science and Technology, University of Shanghai for Science and Technology