Li Weijie, Wang Lihong, Liu Baolin, et al. Experimental Study on the Cooling Rate of a New Type of Oocyte Carriers[J]. Journal of refrigeration, 2016, 37(1).
DOI:
Li Weijie, Wang Lihong, Liu Baolin, et al. Experimental Study on the Cooling Rate of a New Type of Oocyte Carriers[J]. Journal of refrigeration, 2016, 37(1). DOI: 10.3969/j.issn.0253-4339.2016.01.114.
Experimental Study on the Cooling Rate of a New Type of Oocyte Carriers
Cooling rate is the key factor that determines the cryopreserving effect of cells
which has much to do with the forms and the usages of freezing carrier. In this study
digital oscilloscopes and type T thermocouple of 80 μm diameter were employed to establish a high speed temperature measurement system. The cooling rates of cells were measured by using a new type of enclosed oocyte freezing carrier in different ways of being inserted from air to liquid nitrogen. The results showed that the cooling of freezing carrier is mainly fulfilled via heat convection; When the carriers were horizontally placed
the cooling rate of carrier when the air hole was perpendicular to liquid nitrogen is higher than that when the air hole was tangent to liquid nitrogen; when the freezing carrier was directly inserted into liquid nitrogen
the cooling rate of carrier was higher than that when the freezing carrier was inserted into liquid nitrogen and then swayed; when the freezing carrier was placed downwards
the cooling rate of carrier was higher than that vertically or horizontally placed. Based on the results mentioned above
when the carrier was directly inserted into liquid nitrogen downwards
the cooling rate could reach as high as 54572 ± 1338 K/min
which is the highest and favorable to verification of oocytes.