In order to study the relationship between cryoprotective and its molecular structure

Pyris-diamond DSC was used in the experiment to study the physical properties of seven kinds of cryoprotective agent

including phase transition temperature

the area melting peaks phase

transition enthalpy value and hydration properties. The unfrozen water share of cryoprotectant was calculated by cooling curve of the phase transition temperature and that of the phase transition enthalpy value. We have discovered that there is a positive correlation between the protective effect of cryoprotectant and the linear arrangement of hydroxyl; the more hydroxyl in the linear arrangement

the better protective effect of cryoprotectant. As a result

the protective effect of cryoprotectant adding sorbitol solution is the highest in seven kinds of cryoprotective agent because sorbitol has five hydroxy.