Cooling Rate Measurement and System Optimization for Cryotop Device

Li Weijie; Zhou Xinli; Dai Jianjun; Zhang Defu; Liu Baolin; Wang Haisong

doi:10.3969/j.issn.0253-4339.2013.05.095

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Cooling Rate Measurement and System Optimization for Cryotop Device

更新时间：2024-07-18

- Cooling Rate Measurement and System Optimization for Cryotop Device
- Journal of Refrigeration Vol. 34, Issue 5, (2013)
- 作者机构：
  
  1. 上海理工大学生物热科学研究所
  2. 上海农业科学院畜牧兽医研究所上海农业遗传育种重点实验室动物遗传工程研究室上海
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0253-4339.2013.05.095
  CLC：
- Published：2013
- 稿件说明：
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Li Weijie, Zhou Xinli, Dai Jianjun, et al. Cooling Rate Measurement and System Optimization for Cryotop Device[J]. Journal of refrigeration, 2013, 34(5).
DOI：

Li Weijie, Zhou Xinli, Dai Jianjun, et al. Cooling Rate Measurement and System Optimization for Cryotop Device[J]. Journal of refrigeration, 2013, 34(5). DOI： 10.3969/j.issn.0253-4339.2013.05.095.

摘要

Cryotop法是玻璃化保存卵母细胞较有效的方法之一。当Cryotop载体插入液氮时，降温速度极高，几乎瞬间完成，常规的方法无法测量。实验使用数字示波器、线径25μm 的T型热电偶建立起高速测温系统，测量了Cryotop法用于卵母细胞低温保存时的降温速率。通过改变载体材料、厚度、冷源温度等条件，提高Cryotop的降温速率。结果发现当使用60μm的铜质载板，使用浆状液氮作为冷源时，降温速度最高，可达到37130±1336 K/min，较商品化的Cryotop的降温速度（11982±1936 K/min）提高了2倍。

Abstract

Cryotop is one of the best effective methods for oocytes cryopreservation. When Cryotop is inserted into liquid nitrogen

the cooling speed is extremely high

which is difficult to measure by conventional method. In this study

digital oscilloscopes and type T thermocouple of 25 μm diameter were employed to establish a high speed temperature measurement system. The cooling rates of Cryotop device at different carrier material

thickness and cold source temperature were measured. The results showed that when 60μm copper was used as carrier and slush nitrogen was used as cold source

the cooling rate was increased. It could reach 37130 ±1336 K/min

which was twice higher than that of commercially available Cryotop (11982 ± 1936 K/min).

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Related Institution

Tianjin Key Laboratory of Refrigeration Technology，Tianjin University of Commerce

中国科学技术大学工程科学学院

合肥工业大学汽车与交通工程学院

Tianjin Key Lab of Refrigeration Technology, Tianjin University of Commerce

Institue of Biothermal Science and Technology, University of Shanghai for Science and Technology

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