To study the effects of Hydroxyapatite (HA) nanoparticles on devitrification of different cryoprotectant solutions

the crystallization of Glycerol(60%

w/w) and PEG-600(50%

w/w) solutions with HA nanoparticles of different particle size(20nm、40nm、60nm）and concentrations（0.1%、0.5%）were observed under a cryomicroscope. Experimental results showed that the morphology of Glycerol and PEG-600 solutions is dendritic and spheric respectively

and both structures are not changed by adding nanoparticles. The ice fraction of Glycerol solution containing 0.5% HA with the particle size of 60nm is 1/7 of that of Glycerol solution without nanoparticles. The ice fraction of PEG-600 solution increased drastically between -64℃ and -52℃

and the ice fraction of PEG-600 solution containing 0.5% HA with the particle size of 40nm increased by 90%. Ice growth rate has two maximum normally

however

there is only one maximum in Glycerol solution containing 0.5% HA with the particle size of 40nm

PEG-600 solution with the particle size of 60nm

and PEG-600 solution without nanoparticles.