Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs

Yang Bo; Shen Li; Huang Danhua; Liu Baolin

doi:10.3969/j.issn.0253-4339.2012.03.062

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Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs

更新时间：2024-07-18

- Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs
- Journal of Refrigeration Vol. 33, Issue 3, (2012)
- 作者机构：
  
  上海理工大学医疗器械与食品学院
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0253-4339.2012.03.062
  CLC：
- Published：2012
- 稿件说明：
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Yang Bo, Shen Li, Huang Danhua, et al. Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs[J]. Journal of refrigeration, 2012, 33(3).
DOI：

Yang Bo, Shen Li, Huang Danhua, et al. Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs[J]. Journal of refrigeration, 2012, 33(3). DOI： 10.3969/j.issn.0253-4339.2012.03.062.

摘要

将传统肝细胞冻存保护剂配方中的DMSO的浓度减为5%，并添加不同浓度的D-山梨醇、木糖醇或麦芽糖醇，置于-80℃的冰箱冻存两周，两周后将细胞快速复温，进行细胞存活率、24h贴壁率以及细胞形态学的检测，并与10%浓度DMSO组对照比较。结果表明， D-山梨醇、木糖醇、麦芽糖醇均对人肝细胞的低温保存有一定的保护作用，其中5%DMSO+0.4M D-山梨醇组复温后细胞的存活率、贴壁率均优于其他冻存液组，D-山梨醇与DMSO联合使用降低了冻存保护剂DMSO的浓度，表明两者有协同作用。

Abstract

Different types of sugar alcohol is usually used as cryoprotective agents (CPAs) to preserve human hepatocytes. In this study

a new types of sugar alcohol based CPAs with low toxicity was explored by replacing Me2SO with D-sorbic alcohol

xylosic alcohol and maltol. The Me2SO concentration in CPAs was reduced to 5%. Human hepatocytes attached to plastic cover slips were treated with the above CPAs and then placed in the fridge at -80℃ for two weeks. The survival rate

24h cell detachment and cell morphology were tested and compared with the results using 10% Me2SO as CPAs. The results show that 5% Me2SO with D-sorbic alcohol gives the best results. It is concluded that the new CPAs are effective to human hepatocytes cryopreservation.

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Related Institution

Institute of Biothermal Science and Technology, University of Shanghai for Science and Technology

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上海理工大学

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