Research of Enzyme-labeled Methamidophos Cryopreservation for Enzyme Thermal Sensor

Nguyen Van-luc; Xu Fei; Yu Jinsong; Hua Zezhao

doi:10.3969/j.issn.0253-4339.2012.01.051

您当前的位置：

首页 >

文章列表页 >

Research of Enzyme-labeled Methamidophos Cryopreservation for Enzyme Thermal Sensor

更新时间：2024-07-18

- Research of Enzyme-labeled Methamidophos Cryopreservation for Enzyme Thermal Sensor
- Journal of Refrigeration Issue 1, (2012)
- 作者机构：
  
  上海理工大学低温与食品冷冻研究所
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0253-4339.2012.01.051
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
Nguyen Van-luc, Xu Fei, Yu Jinsong, et al. Research of Enzyme-labeled Methamidophos Cryopreservation for Enzyme Thermal Sensor[J]. Journal of refrigeration, 2012, (1).
DOI：

Nguyen Van-luc, Xu Fei, Yu Jinsong, et al. Research of Enzyme-labeled Methamidophos Cryopreservation for Enzyme Thermal Sensor[J]. Journal of refrigeration, 2012, (1). DOI： 10.3969/j.issn.0253-4339.2012.01.051.

摘要

采用加速实验中获得的较优保护剂配方（2.0mol/L氯化钠、4.0mol/L木糖醇、1.25mol/L甘油），对酶热传感器中酶标甲胺磷农药低温贮存过程中的酶活变化进行了研究。结果显示：加或未加复合保护剂时，酶标农药的贮存温度与酶活损失速率常数之间符合Arrhenius关系，且酶标农药-18℃条件下贮存6个月后，酶活保留率分别为77.13%（未加保护剂）和97.44%（加保护剂）。与化学动力学方法获得的理论预测值相比，相对标准偏差分别为3.10%（未加保护剂）和0.51%(加保护剂)。这一结果将为下一步的农残检测酶热传感器的开发奠定基础。

Abstract

Cryopreservation of enzyme-labeled methamidophos was studied using the composite protective agent (2.0mol /L NaCl

4.0mol/L xylitol and 1.25mol/L glycerol) obtained from accelerated test. The results show that the relationship between enzyme activity loss rate and storage temperature meet arrhenius equation. After 6 months

the retention rate of enzyme activity is 77.13%(without protective agents) and 97.44% (with protective agents) under the storage conditions of -18℃. Comparing to theoretical prediction value according to chemical kinetics

the relative standard deviation was 3.10% (without protective agents) and 0.51% (with protective agents)

respectively. The result will lay the foundation for the development of thermal pesticide residue biosensor.

关键词

Keywords

references

Views

2139

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

An Investigation on Stability and Sintering Property of LiBr Aqueous Solution Added Nano-particles and Relevant Dispersants

Research on Control Stability of Cascade Air-source Heat Pump

Study on Stability and Boiling Temperature of Nano-LiBr Aqueous Solution

Experimental Investigation on Instability of Linear Compressor in Refrigerator

Related Author

Xie Guozhen

Zhang Xinxing

Wang Gang

Chen Jianbo

Yao Jingshan

Kang Youli

Han Xing

Li He

Related Institution

Beijing University of Civil Engineering and Architecture

School of Environment and Architecture, University of Shanghai for Science and Tecnolgy

CCDI GROUP

School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture

Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry

Address：10/F, Yindu Mansion, 67 Fucheng Road, Haidian Dist, Beijing, China Postal code：100142
Tel：010-68711412 Email：editor@car.org.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰