Cryomicroscope is an important tool in the study of cryopreservation of biomaterials. The accuracy of temperature measurement is influenced by the location of the thermocouple and the volume of the sample. The temperature distribution on the cryostage was numerically simulated and verified with experiments. The results show that the temperature of the sample can be estimated from the measured value of the thermocouple placed on top of the cover glass

and the planar temperature distribution of the cryostage is approximately uniform.