| 摘要: |
| 在单层贴壁细胞的慢速冻存中，细胞从基质上脱落是影响冻存效果的的主要因素之一。造成这种脱落的主要原因是基质和细胞的热膨胀系数差异，而降温速率和终温均为影响热膨胀系数变化的因素。本文通过测量肝细胞及微载体在慢速冷冻过程中（降温速率分别为1、2和5 ℃/min，终温为﹣30 ℃）热膨胀系数，对比筛选出热膨胀差异最小时的冻存方案，研究热膨胀损伤对肝细胞复合体冻存效果的影响。实验结果显示：降温速率为1 ℃/min时，肝细胞与微载体间的热膨胀差异最小，对应的复合体冻存效果最好，其存活率和贴壁率分别为62.66%±0.67%和37.2%±1.25%。细胞与微载体间的热膨胀损伤存在于整个降温过程，是影响肝细胞复合体冻存效果的主要因素之一。 |
| 关键词: 低温保存 热膨胀损伤 肝细胞-微载体复合体 低温显微镜 |
| DOI： |
| 投稿时间：2020-12-15 修订日期：2021-04-04
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| 基金项目:国家自然科学基金（51776130）资助项目。 |
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| Study on the Mechanism of Thermal Expansion Injury of Hepatocyte Microspheres during Cryopreservation |
| Yu Zengying,Liu Baolin |
| (Institute of Biothermal and Technology，University of Shanghai for Science and Technology) |
| Abstract: |
| In the cryopreservation of monolayer adherent cells, the detachment of cells from the substrate is one of the main factors that influence the effect of cryopreserved cells. The main reason for this type of shedding is the difference in the coefficient of thermal expansion (CTE) of the matrix and cells. The cooling rate and final temperature are both factors that cause a change in the CTE. In this study, the cryopreservation plan was compared and screened when the difference in thermal expansion was the smallest. The effect of thermal expansion damage on the cryopreservation of hepatocyte complexes was explored by measuring the CTE of hepatocytes and microcarriers during the slow freezing process (cooling rate was 1 ℃/min, 2 ℃/min, and 5 ℃/min, and the final temperature of was -30 ℃. The final experiment concluded that when the cooling rate was 1 ℃/min, the thermal expansion difference between hepatocytes and microcarriers was the smallest, and the cryopreservation effect of the corresponding composite was the best, with survival and adhesion rates of 62.66%±0.67% and 37.2%±1.25%, respectively. Thermal expansion damage between cells and microcarriers exists throughout the cooling process and is one of the main factors affecting the cryopreservation effect of liver cell complexes. |
| Key words: cryopreservation thermal expansion damage hepatocyte-microcarrier complex cryogenic microscope |
