| 摘要: |
| 外界的剧烈变化会对细胞内部产生一定的影响，本文通过考马斯亮蓝染色法研究细胞骨架中的微丝在脱水过程中的形态变化。通过图像处理的方法对骨架图像的灰度峰度进行分析处理，发现随着脱水过程的进行，灰度图像出现弥散性下降，骨架形态更为疏松并伴随解聚等一系列形态变化。对图像的特征参数进行定量计算后，结果表明：持续真空环境下骨架的最终相对变化率为32.7%，持续低温环境下的最终相对变化率为59%。对细胞变形度研究结果表明，脱水过程会对细胞骨架产生较为显著的影响。 |
| 关键词: 细胞生物学 骨架 形态学 真空度 冷却速率 显微结构 |
| DOI： |
| 投稿时间：2019-07-02 修订日期：2019-10-09
|
| 基金项目:国家自然科学基金(51676139)资助项目 |
|
| Effects of Dehydration on Cytoskeleton |
| Zhu Kai,Dong Yang,Wang Yabo,Xie Yanqi |
| (Tianjin Key Laboratory of Refrigeration Technology, Tianjin University of Commerce) |
| Abstract: |
| Drastic changes in the surrounding environment have a certain effect on the inner regions of cells. In this study, the morphology of microfilaments in the cytoskeleton during dehydration is studied by Coomassie brilliant blue staining and digital image processing. By analyzing the gray-scale kurtosis of the processed image, it is observed that as the dehydration process progresses, the gray-scale image shows a decrease in dispersion, the skeleton morphology is looser, and a series of morphological changes are accompanied by depolymerization. Through quantitative calculation of the characteristic parameters of the image, it is inferred that the final relative rate of change of the skeleton under a continuous vacuum environment is 32.7%, and that under a continuous low temperature environment is 59%. Finally, a study on the degree of cell deformation shows that the dehydration process has a more significant effect on the cytoskeleton. |
| Key words: cell biology skeleton morphology vacuum degree cooling rate microstructure |
