| 摘要: |
| 在低温保存领域，玻璃化能有效防止冰晶的形成，具有良好的保存效果。玻璃化保存需要的条件是极快的降温速率和高浓度的低温保护剂，但是高浓度低温保护剂会对细胞造成渗透损伤和毒性损伤。最小体积法能通过减小样本体积，大大提高降温速率，降低实现玻璃化所需的保护剂浓度。本文综述了最小体积法玻璃化保存的最新研究进展，包括有载体的微滴玻璃化保存、喷射微滴玻璃化保存、生物打印微滴玻璃化保存和微流体封装微滴玻璃化保存。其中，喷射微滴玻璃化保存，生物打印微滴玻璃化保存及微流体封装微滴玻璃化保存，是将新型的微滴产生技术与玻璃化技术相结合，因其能迅速产生大小均匀的微滴，具有高通量特点，在低温保存领域具有很大的发展潜力。 |
| 关键词: 最小体积法 玻璃化 喷射微滴 生物打印 微流体封装 |
| DOI： |
| 投稿时间：2017-08-09
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| 基金项目:国家自然科学基金( 51376132)。 |
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| Research Progresses in Vitrification by Minimum Volume Method |
| Zhang Xiaomin,Zhou Xinli |
| (School of Medical Instrument and Food Engineering，University of Shanghai for Science and Technology) |
| Abstract: |
| Vitrification is an effective method in the field of cryopreservation since it can prevent the formation of ice crystals. Vitrification requires ultra-fast cooling rates and high concentrations of cryoprotective agents. However, osmotic injury and toxic damage can be caused by high concentration of cryoprotective agents. The minimum volume methods can significantly increase the cooling rate and reduce the concentration of cryoprotective agents in vitrification. In this paper, the latest research progresses in vitrification by minimum volume methods are reviewed. The minimum volume methods include carrier-based droplet vitrification, spray droplet vitrification, bio-printing droplet vitrification, and microfluidic encapsulation droplet vitrification. Among these, spray droplet vitrification, bio-printing droplet vitrification, and microfluidic encapsulation droplet vitrification are the combination of emerging droplet generation technology and vitrification technology. Because of the rapid generation of uniform droplets and high throughput, they have great potential in the field of cryopreservation. |
| Key words: minimum volume methods vitrification spray droplet bio-printing microfluidic encapsulation |
